A NEW RP-HPLC METHOD FOR ESTIMATION OF VERICIGUAT IN BULK DRUG AND PHARMACEUTICAL DOSAGE FORM

Abstract

A simple, rapid, specific and accurate reverse phase high performance liquid chromatographic method has beendeveloped for the validation of Vericiguat in bulk as well as in marketed pharmaceutical dosage form. This separation wasperformed on a Symmetry ODS C18 (4.6×250mm, 5μm) column with Methanol:Phosphate Buffer (55:45) v/v asmobile phase at a flow rate of 1.0 mL min−1 with UV detection at 225 nm; the constant column temperature wasambient. The run time under these chromatographic conditions was less than 8 min. The retention time of Vericiguatwas found to be 2.252. The calibration plot was linear over the concentration range of 6 - 14 μg mL−1 with limits ofdetection and quantification values of 1.2 and 3.6 μg mL−1 respectively. The mean % assay of marketed formulation wasfound to be 99.86%, and % recovery was observed in the range of 98-102%. Relative standard deviation for theprecision study was found <2%. The developed method was simple, precise, specific, accurate and rapid, making itsuitable for estimation of Vericiguat in bulk and marketed pharmaceutical dosage form dosage form.