Acute necrotizing pneumonia and pyofibrinothorax in an experiment

Abstract

Nowadays, the problem of diagnosis and treatment of purulent-destructive diseases of the bronchopulmonary system in children remains relevant, that is connected with a large number of pulmonary-pleural forms and complications of acute necrotizing pneumonia. Purpose - to establish the correlation of changes in lung tissue and the pleural cavity of a growing organism, depending on the causative agents of pneumonia, elucidation and study of the mechanisms of pyofibrothorax formation and development. Materials and methods. The characteristic features of histopathological tissue changes in the lung parenchyma and adjacent tissues have been analyzed (visceral and parietal pleura, adhesion formation sections) of 45 immature laboratory rats, divided into 5 groups: the control group (intact animals), the Group 2 - contaminated with Klebsiella pneumoniae, the Group 3 - Staphylococcus aureus, the Group 4 - Candida albicans and the Group 5 with mixed flora (Klebsiella pneumoniae+Staphylococcus aureus) adding fungal infection (Candida albicans). Results. It was proved that the formation of a massive pyofibrinothorax is enhanced by mixed flora with the addition of fungi, that is more pronounced in the fifth series (group) of the experiment. Taking into account the data obtained experimentally, we consider whether fungi are Lukianenkoobtained in cultures of patients’ flora, they should be classified as a risk group of fibrothorax formation. Conclusions. Morphological changes of tissues under the influence of the infectious agent reached their maximum on the ninth day of the experiment (p<0.05). The pattern of necrotizing pneumonia is more pronounced in the Group 5 of experimental animals, which morphologically manifests itself in a more massive inflammatory infiltration (mostly due to rod- and segmented-nuclear neutrophils), foci of destruction and abscessation of the lung parenchyma and has a direct association with the polymicrobial etiology of the disease (Klebsiella pneumoniae+Staphylococcus /taureus+Candida albicans) (p<0.05). The formation of a massive pyofibrinothorax is enhanced by mixed flora, especially with the addition of fungi. The study was carried out in accordance with the principles of the European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes, as well as the law of Ukraine. The research protocol was approved by the Local Ethics Committee of the institution mentioned in the article. No conflict of interests was declared by the authors.