Effect of melatonin on oxidative stress of differentiated Dopaminergic cells

IF 0.7 Q4 PHARMACOLOGY & PHARMACY Egyptian Pharmaceutical Journal Pub Date : 2023-07-01 DOI:10.4103/epj.epj_22_23
H. Ibrahim, Abdelaziz M. Hussein, Mahmoud Gabr, Abdel A.F., Rasha El-Saeed
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Abstract

Background Although stem cells therapies provide a great deal in the treatment of several disease, they lack their normal functions after transplantation due to inflammation and oxidative stress. Melatonin has a powerful antioxidant ability and can enhance the effect of stem cells. Objectives This work aimed to investigate the melatonin’s effect on oxidative stress of differentiated adipose-mesenchymal stem cells (AD-MSCs) to dopaminergic (DAergic) cells. Material and methods The AD-MSCs cells were characterized after passage 3 by flow cytometry method and divided into four groups: (a) control group that was nontreated AD-MSCs, (b) MSCs+M group that was AD-MSCs cultured with 1 μM melatonin in expansion media for 12 days, (c) DN group that was MSCs treated with neurobasal A media for 12 days, (d) DN+M group which was MSCs cultured with 1 μM melatonin and neurobasal A media for 12 days. After 12 days, the catalase (CAT) activity and malondialdehyde (MDA) level were measured by using ELISA. Also, the gene expression level of MAP-2 was detected. Results and conclusion The current study proved that the isolated cells were MSCs due to high expression percentages for CD73 and CD90 and low expression percentages for CD34 and CD45. The DN+M group showed the highest expression of MAP-2 gene when compared to the other different groups (P ≤ 0.05). Moreover, there was a significant increase in CAT concentration in groups treated with melatonin than other group (P ≤ 0.05), while, there was no change in MDA level between all groups. It was concluded that melatonin has an effective antioxidative role throughout the differentiation process of AD-MSCs into DAergic neural cells
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褪黑激素对分化多巴胺能细胞氧化应激的影响
背景 虽然干细胞疗法在治疗多种疾病方面发挥了巨大作用,但由于炎症和氧化应激,干细胞在移植后缺乏正常功能。褪黑素具有强大的抗氧化能力,可增强干细胞的作用。目的 本研究旨在探讨褪黑素对已分化的脂肪间充质干细胞(AD-MSCs)转化为多巴胺能(DAergic)细胞的氧化应激的影响。材料与方法 AD-MSCs细胞经过3次分化后,用流式细胞仪进行表征,并分为四组:(a)对照组,即未经处理的AD-MSCs;(b)MSCs+M组,即用1 μM褪黑素在扩增培养基中培养12天的AD-MSCs;(c)DN组,即用神经节肽A培养基处理12天的MSCs;(d)DN+M组,即用1 μM褪黑素和神经节肽A培养基培养12天的MSCs。12 天后,用酶联免疫吸附法测定过氧化氢酶(CAT)活性和丙二醛(MDA)水平。此外,还检测了 MAP-2 的基因表达水平。结果与结论 目前的研究证明,分离出的细胞是间充质干细胞,因为其 CD73 和 CD90 的表达率较高,而 CD34 和 CD45 的表达率较低。与其他不同组相比,DN+M 组的 MAP-2 基因表达量最高(P ≤ 0.05)。此外,褪黑素治疗组的 CAT 浓度比其他组明显增加(P ≤ 0.05),而各组之间的 MDA 水平没有变化。结论是褪黑素在 AD-MSCs 向 DAergic 神经细胞分化的整个过程中具有有效的抗氧化作用。
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来源期刊
Egyptian Pharmaceutical Journal
Egyptian Pharmaceutical Journal PHARMACOLOGY & PHARMACY-
CiteScore
1.10
自引率
0.00%
发文量
37
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