Influence of date palm pollen grain extract on rabbit buck semen characteristics throughout chilled storage period of 72 h

IF 0.7 Q4 PHARMACOLOGY & PHARMACY Egyptian Pharmaceutical Journal Pub Date : 2023-07-01 DOI:10.4103/epj.epj_15_23
I. El-Seadawy, Mohamed Kotp, Heba Hozyen, W. El-Nattat, M. El-Tohamy
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Abstract

Background The demand for using stored semen in artificial insemination programs of livestock animals is increasing. Therefore, developing and improving methods for semen preservation would provide adequate fertility rates that maintain the high production rates for rabbit industry. Several studies on preservation protocols and extender composition have been carried out. Objective The current study was designed to examine the effect of various concentrations of date palm pollen grain extract (DPPE) on postchilling quality of rabbit semen. Materials and methods Total phenolic and flavonoid substances and antioxidant activity were assessed in DPPE. High-performance liquid chromatography was used for identification and separation of goal metabolites. Semen was gathered from 10 male rabbits, grouped, and then split into five fractions (500 μl each). The first fraction represented as control, whereas DPPE was supplemented at concentrations of 1.6, 2.0, 2.4, and 2.8 mg/5 ml tris-citric extender. Extended semen specimens were cooled at 4°C for 72 h. Motile, life, abnormal, membrane, and acrosome integrity percentages of sperms were appraised in chilled semen all over the refrigeration period. Results and conclusion Total phenolic and total flavonoids contents in the DPPE were 4.15 mg GAE/g extract and 0.74 mg CE/g extract, respectively. The DPPE specimen showed various antiradical activity gauged toward 2,2-azino-bis/3-ethil-benothiazoline-6-sulfonic acid (12.37 mM TE/g) and 2,2-Diphenyl-1-picryl-hydrazyl (4.06 mM TE/g). However, the reducing capacity assessed by ferric reducing activity power method was 9.19 mM TE/g/g. The most effective compounds in the DPPE were pyrogallol (4150.92 μg/g extract), ferulic acid (2935.50 μg/g extract), and rutin (2163.99 μg/g extract). The enrichment of semen extenders with 2.4 mg DPPE/5 ml tris-citric extender had preserved the sperm forward motility, sperm livability, sperm acrosome integrities, and sperm membrane integrities in an upright state during cooling till 72 h related to control treating. No adverse effects were recorded on sperm abnormalities. It could be concluded that the enriching of rabbit bucks’ semen tris-basic extender by 2.4 mg DPPE/5 ml tris-extender (the perfect and harmless concentration) sustained the sperm features in decent conditions all over a cooling period of 72 h.
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枣椰花粉萃取物在 72 小时冷藏期间对家兔精液特征的影响
背景 在家畜人工授精计划中使用储存精液的需求日益增加。因此,开发和改进精液保存方法可提供足够的受精率,从而维持养兔业的高生产率。有关精液保存方案和延长剂成分的研究已经开展了多项。本研究旨在探讨不同浓度的枣椰花粉颗粒提取物(DPPE)对兔精液冷藏后质量的影响。材料和方法 评估 DPPE 中的总酚和类黄酮物质以及抗氧化活性。采用高效液相色谱法鉴定和分离目标代谢物。收集 10 只雄性兔子的精液,分组后分成 5 份(每份 500 μl)。第一个馏分为对照组,而 DPPE 的补充浓度分别为 1.6、2.0、2.4 和 2.8 mg/5 ml 三柠檬酸扩展剂。在整个冷藏期间,对冷藏精液中精子的活动率、寿命、畸形率、膜完整性和顶体完整性进行评估。结果与结论 DPPE 中的总酚和总黄酮含量分别为 4.15 毫克 GAE/克提取物和 0.74 毫克 CE/克提取物。DPPE 试样对 2,2-叠氮-双/3-乙二基-苯并噻唑啉-6-磺酸(12.37 mM TE/g)和 2,2-二苯基-1-苦基肼(4.06 mM TE/g)具有不同的抗自由基活性。然而,用铁还原活性力法评估的还原能力为 9.19 mM TE/g/g。DPPE 中最有效的化合物是焦棓醇(4150.92 μg/g 提取物)、阿魏酸(2935.50 μg/g 提取物)和芦丁(2163.99 μg/g 提取物)。与对照组相比,用 2.4 毫克 DPPE/5 毫升三柠檬酸扩展剂对精液扩展剂进行富集后,精子的前向运动能力、精子活率、精子顶体完整率和精子膜完整率在冷却至 72 小时后仍保持直立状态。未发现精子异常的不良影响。由此可以得出结论,用 2.4 毫克 DPPE/5 毫升三碱式扩展剂(最佳和无害浓度)对家兔精液三碱式扩展剂进行浓缩,可以在 72 小时的冷却期内保持精子的正常特征。
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来源期刊
Egyptian Pharmaceutical Journal
Egyptian Pharmaceutical Journal PHARMACOLOGY & PHARMACY-
CiteScore
1.10
自引率
0.00%
发文量
37
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