The Effects of Tiger Milk Mushroom Lignosus rhinocerus TM02® (Agaricomycetes) on Leukaemogenic Tyrosine Kinase Cell Lines

IF 1.4 4区生物学 Q4 MYCOLOGY International journal of medicinal mushrooms Pub Date : 2024-01-01 DOI:10.1615/intjmedmushrooms.2024052325

Norhaida Che Azmi, Tuan Syaripah Atiqah Najwa Tuan Yahya, Fung Shin Yee, Ng Szu Ting, Tan Chon Seng

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Abstract

Leukemia can be a result of genetic changes associated with protein tyrosine kinase activity such as in MPL W515L and BCR/ABL genes. However, the current conventional treatment of leukemia produces severe side effects that urge the approach to use natural products. A medicinal mushroom, Lignosus rhinocerus shows potential as an anti-cancer treatment. To investigate the efficacy and mechanism of action of the L. rhinocerus cultivar (TM02®) extract on leukaemogenic tyrosine kinase cell lines, a cold-water extract (CWE) was produced by using TM02® sclerotia powder at 4◦C. The carbohydrate and protein contents were found to be 77.24% and 1.75% respectively. In comparison to the normal Ba/F3 cell, the CWE TM02® shows significant effects on exhibiting proliferation of Ba/F3 expressed MPL W515L and BCR/ABL, possibly due to the presence of phenolic compounds and antioxidant properties of TM02®, which contribute to act on various signalling pathways, and the reported apoptotic activity of CWE TM02®. In contrast, CWE TM02® significantly exhibited high scavenging activity of both Ba/F3 expressed MPL W515L and BCR/ABL. At concentrations of 125 µg/mL and 500 µg/mL of CWE TM02® decreased 49.5% and 67.5% of cell migration activity of Ba/F3 expressed MPL W515L and BCR/ABL respectively. Therefore, we postulate that CWE TM02® has the capability to mediate the migration route of the leukaemogenic tyrosine kinase cell lines.

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虎乳菇 Lignosus rhinocerus TM02®（姬松茸）对白血病酪氨酸激酶细胞株的影响

白血病可能是与蛋白酪氨酸激酶活性有关的基因变化所致，如 MPL W515L 和 BCR/ABL 基因。然而，目前治疗白血病的传统方法会产生严重的副作用，这促使人们开始使用天然产品。一种药用蘑菇 Lignosus rhinocerus 具有抗癌治疗的潜力。为了研究犀牛菌栽培品种（TM02®）提取物对白血病酪氨酸激酶细胞系的疗效和作用机制，我们在 4◦C 温度下使用 TM02® 硬菌粉生产了冷水提取物（CWE）。碳水化合物和蛋白质含量分别为 77.24% 和 1.75%。与正常的 Ba/F3 细胞相比，CWE TM02® 对表达 MPL W515L 和 BCR/ABL 的 Ba/F3 细胞的增殖有显著影响，这可能是由于 TM02® 中含有酚类化合物和抗氧化特性，有助于作用于各种信号通路，以及据报道 CWE TM02® 具有凋亡活性。相比之下，CWE TM02®对Ba/F3表达的MPL W515L和BCR/ABL都有明显的高清除活性。浓度为 125 µg/mL 和 500 µg/mL 的 CWE TM02® 可使 Ba/F3 表达的 MPL W515L 和 BCR/ABL 的细胞迁移活性分别降低 49.5% 和 67.5%。因此，我们推测 CWE TM02® 有能力介导致白血病酪氨酸激酶细胞株的迁移途径。

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来源期刊

International journal of medicinal mushrooms 医学-药学

CiteScore

2.60

自引率

16.70%

发文量

审稿时长

6-12 weeks

期刊介绍： The rapid growth of interest in medicinal mushrooms research is matched by the large number of disparate groups that currently publish in a wide range of publications. The International Journal of Medicinal Mushrooms is the one source of information that will draw together all aspects of this exciting and expanding field - a source that will keep you up to date with the latest issues and practice. The International Journal of Medicinal Mushrooms published original research articles and critical reviews on a broad range of subjects pertaining to medicinal mushrooms, including systematics, nomenclature, taxonomy, morphology, medicinal value, biotechnology, and much more.