Tissue clearing and its applications in human tissues: A review

IF 9.7 4区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS VIEW Pub Date : 2024-01-15 DOI:10.1002/viw.20230046
Hongcheng Mai, Dan Lu
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Abstract

In three-dimensional (3D) space, an unbiased and systemic view of human specimens between structures and functions is required. However, conventional histological sections from specimens have made only limited progress in exploring intact information about 3D biological tissues. With significant advances in optical physics and chemical engineering, state-of-the-art tissue-clearing methods can revolutionize the intact subcellular level of human tissue histological analysis, from thick human tissues to intact human organs. The present review summarizes the principle of tissue clearing so that a trainee researcher can implement effective human tissue-clearing protocols. Furthermore, this review highlights existing tissue-clearing methods in specific human tissue applications, describes imaging strategies, and presents various efficient computational approaches for processing and visualizing large image data. Finally, potential future directions for developing tissue-clearing methods for human tissues are also discussed.

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组织清除及其在人体组织中的应用:综述
在三维(3D)空间中,需要对人体标本的结构和功能进行无偏见的系统观察。然而,传统的标本组织学切片在探索三维生物组织的完整信息方面进展有限。随着光学物理和化学工程的长足进步,最先进的组织清理方法可以彻底改变人体组织组织学分析的完整亚细胞水平,从厚厚的人体组织到完整的人体器官。本综述总结了组织清理的原理,以便实习研究人员能够实施有效的人体组织清理方案。此外,本综述还重点介绍了特定人体组织应用中的现有组织清理方法,描述了成像策略,并介绍了处理和可视化大型图像数据的各种高效计算方法。最后,还讨论了开发人体组织清除方法的潜在未来方向。
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来源期刊
VIEW
VIEW Multiple-
CiteScore
12.60
自引率
2.30%
发文量
0
审稿时长
10 weeks
期刊介绍: View publishes scientific articles studying novel crucial contributions in the areas of Biomaterials and General Chemistry. View features original academic papers which go through peer review by experts in the given subject area.View encourages submissions from the research community where the priority will be on the originality and the practical impact of the reported research.
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