Quantification of cellular phototoxicity of organelle stains by the dynamics of microtubule polymerization

IF 9.7 4区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS VIEW Pub Date : 2024-08-22 DOI:10.1002/viw.20240013
Shivam Mahapatra, Seohee Ma, Bin Dong, Chi Zhang
{"title":"Quantification of cellular phototoxicity of organelle stains by the dynamics of microtubule polymerization","authors":"Shivam Mahapatra, Seohee Ma, Bin Dong, Chi Zhang","doi":"10.1002/viw.20240013","DOIUrl":null,"url":null,"abstract":"Being able to quantify the phototoxicity of dyes and drugs in live cells allows biologists to better understand cell responses to exogenous stimuli during imaging. This capability further helps to design fluorescent labels with lower phototoxicity and drugs with better efficacy. Conventional ways to evaluate cellular phototoxicity rely on late-stage measurements of individual or different populations of cells. Here, we developed a quantitative method using intracellular microtubule polymerization as a rapid and sensitive marker to quantify early-stage phototoxicity. Implementing this method, we assessed the photosensitization induced by organelle dyes illuminated with different excitation wavelengths. Notably, fluorescent markers targeting mitochondria, nuclei, and endoplasmic reticulum exhibited diverse levels of phototoxicity. Furthermore, leveraging a real-time precision opto-control technology allowed us to evaluate the synergistic effect of light and dyes on specific organelles. Studies in hypoxia revealed enhanced phototoxicity of Mito-Tracker Red CMXRos that is not correlated with the generation of reactive oxygen species but a different deleterious pathway in low oxygen conditions.","PeriodicalId":34127,"journal":{"name":"VIEW","volume":null,"pages":null},"PeriodicalIF":9.7000,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"VIEW","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1002/viw.20240013","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
引用次数: 0

Abstract

Being able to quantify the phototoxicity of dyes and drugs in live cells allows biologists to better understand cell responses to exogenous stimuli during imaging. This capability further helps to design fluorescent labels with lower phototoxicity and drugs with better efficacy. Conventional ways to evaluate cellular phototoxicity rely on late-stage measurements of individual or different populations of cells. Here, we developed a quantitative method using intracellular microtubule polymerization as a rapid and sensitive marker to quantify early-stage phototoxicity. Implementing this method, we assessed the photosensitization induced by organelle dyes illuminated with different excitation wavelengths. Notably, fluorescent markers targeting mitochondria, nuclei, and endoplasmic reticulum exhibited diverse levels of phototoxicity. Furthermore, leveraging a real-time precision opto-control technology allowed us to evaluate the synergistic effect of light and dyes on specific organelles. Studies in hypoxia revealed enhanced phototoxicity of Mito-Tracker Red CMXRos that is not correlated with the generation of reactive oxygen species but a different deleterious pathway in low oxygen conditions.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
通过微管聚合动态量化细胞器染色剂的细胞光毒性
通过量化活细胞中染料和药物的光毒性,生物学家可以更好地了解细胞在成像过程中对外源刺激的反应。这种能力还有助于设计光毒性更低的荧光标签和药效更好的药物。评估细胞光毒性的传统方法依赖于对单个或不同细胞群的后期测量。在这里,我们开发了一种定量方法,利用细胞内微管聚合作为快速灵敏的标记来量化早期光毒性。利用这种方法,我们评估了细胞器染料在不同激发波长照射下诱导的光敏作用。值得注意的是,针对线粒体、细胞核和内质网的荧光标记表现出不同程度的光毒性。此外,利用实时精密光控技术,我们还能评估光和染料对特定细胞器的协同效应。在缺氧条件下进行的研究表明,Mito-Tracker Red CMXRos 的光毒性增强与活性氧的生成无关,而是与低氧条件下的不同有害途径有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
VIEW
VIEW Multiple-
CiteScore
12.60
自引率
2.30%
发文量
0
审稿时长
10 weeks
期刊介绍: View publishes scientific articles studying novel crucial contributions in the areas of Biomaterials and General Chemistry. View features original academic papers which go through peer review by experts in the given subject area.View encourages submissions from the research community where the priority will be on the originality and the practical impact of the reported research.
期刊最新文献
Frontiers in artificial intelligence-directed light-sheet microscopy for uncovering biological phenomena and multiorgan imaging Exosomes of immune cell origin and their therapeutic potential for tumors Optical imaging for brown or beige adipose tissue Electrochemical detection of the cardiac biomarker cardiac troponin I Quantification of cellular phototoxicity of organelle stains by the dynamics of microtubule polymerization
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1