A 4D printed self-assembling PEGDA microscaffold fabricated by digital light processing for arthroscopic articular cartilage tissue engineering.

IF 4.4 Q2 ENGINEERING, MANUFACTURING Progress in Additive Manufacturing Pub Date : 2024-01-01 Epub Date: 2022-11-09 DOI:10.1007/s40964-022-00360-0
Yunjie Hao, Chuanyung Wu, Yuchuan Su, Jude Curran, James R Henstock, Fangang Tseng
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Abstract

Articular cartilage in synovial joints such as the knee has limited capability to regenerate independently, and most clinical options for focal cartilage repair merely delay total joint replacement. Tissue engineering presents a repair strategy in which an injectable cell-laden scaffold material is used to reconstruct the joint in situ through mechanical stabilisation and cell-mediated regeneration. In this study, we designed and 3D-printed millimetre-scale micro-patterned PEGDA biomaterial microscaffolds which self-assemble through tessellation at a scale relevant for applications in osteochondral cartilage reconstruction. Using simulated chondral lesions in an in vitro model, a series of scaffold designs and viscous delivery solutions were assessed. Hexagonal microscaffolds (750 μm x 300 μm) demonstrated the best coverage of a model cartilage lesion (at 73.3%) when injected with a 1% methyl cellulose solution. When chondrocytes were introduced to the biomaterial via a collagen hydrogel, they successfully engrafted with the printed microscaffolds and survived for at least 14 days in vitro, showing the feasibility of reconstructing stratified cartilaginous tissue using this strategy. Our study demonstrates a promising application of this 4D-printed injectable technique for future clinical applications in osteochondral tissue engineering.

Supplementary information: The online version contains supplementary material available at 10.1007/s40964-022-00360-0.

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利用数字光处理技术制造的用于关节镜关节软骨组织工程的 4D 打印自组装 PEGDA 显微支架。
膝关节等滑膜关节中的关节软骨独立再生的能力有限,大多数病灶软骨修复的临床方案只是推迟了全关节置换的时间。组织工程学提出了一种修复策略,即使用可注射的含细胞支架材料,通过机械稳定和细胞介导的再生,在原位重建关节。在这项研究中,我们设计并用三维打印了毫米级微图案 PEGDA 生物材料微支架,这种微支架通过细分网格进行自组装,可应用于骨软骨重建。利用体外模型模拟软骨损伤,评估了一系列支架设计和粘性输送解决方案。当注入 1%的甲基纤维素溶液时,六角形微支架(750 μm x 300 μm)对模型软骨病变的覆盖率最高(73.3%)。当软骨细胞通过胶原水凝胶进入生物材料时,它们成功地与打印的微支架接种,并在体外存活了至少 14 天,这表明使用这种策略重建分层软骨组织是可行的。我们的研究表明,这种4D打印注射技术在未来骨软骨组织工程的临床应用中大有可为:在线版本包含补充材料,可查阅 10.1007/s40964-022-00360-0。
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来源期刊
Progress in Additive Manufacturing
Progress in Additive Manufacturing Engineering-Industrial and Manufacturing Engineering
CiteScore
7.20
自引率
0.00%
发文量
113
期刊介绍: Progress in Additive Manufacturing promotes highly scored scientific investigations from academia, government and industry R&D activities. The journal publishes the advances in the processing of different kinds of materials by well-established and new Additive Manufacturing (AM) technologies. Manuscripts showing the progress in the processing and development of multi-materials by hybrid additive manufacturing or by the combination of additive and subtractive manufacturing technologies are also welcome. Progress in Additive Manufacturing serves as a platform for scientists to contribute full papers as well as review articles and short communications analyzing aspects ranging from data processing (new design tools, data formats), simulation, materials (ceramic, metals, polymers, composites, biomaterials and multi-materials), microstructure development, new AM processes or combination of processes (e.g. additive and subtractive, hybrid, multi-steps), parameter and process optimization, new testing methods for AM parts and process monitoring. The journal welcomes manuscripts in several AM topics, including: • Design tools and data format • Material aspects and new developments • Multi-material and composites • Microstructure evolution of AM parts • Optimization of existing processes • Development of new techniques and processing strategies (combination subtractive and additive    methods, hybrid processes) • Integration with conventional manufacturing techniques • Innovative applications of AM parts (for tooling, high temperature or high performance    applications) • Process monitoring and non-destructive testing of AM parts • Speed-up strategies for AM processes • New test methods and special features of AM parts
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