Rodney A Nichols, Amber D Ide, Cody T Morrison, Amber L Anger, Matthew J Buccilli, Cynthia K Damer
{"title":"Copine C plays a role in adhesion and streaming in <i>Dictyostelium</i>.","authors":"Rodney A Nichols, Amber D Ide, Cody T Morrison, Amber L Anger, Matthew J Buccilli, Cynthia K Damer","doi":"10.1080/19336918.2024.2315629","DOIUrl":null,"url":null,"abstract":"<p><p>Copines are a family of calcium-dependent membrane-binding proteins. To study these proteins, anull mutant for <i>cpnC</i> was created in <i>Dictyostelium</i>, which has six copines genes (<i>cpnA-cpnF</i>). During development, <i>cpnC<sup>-</sup></i> cells were able to aggregate, but did not form streams. Once aggregated into mounds, they formed large ring structures. <i>cpnC<sup>-</sup></i> cells were less adherent to plastic substrates, but more adherent to other cells. These phenotypes correlated with changes in adhesion protein expression with decreased expression of SibA and increased expression of CsaA in developing <i>cpnC<sup>-</sup></i> cells. We also measured the expression of RegA, a cAMP phosphodiesterase, and found that <i>cpnC<sup>-</sup></i> cells have reduced RegA expression. The reduced RegA expression in <i>cpnC<sup>-</sup></i> cells is most likely responsible for the observed phenotypes.</p>","PeriodicalId":9680,"journal":{"name":"Cell Adhesion & Migration","volume":"18 1","pages":"1-19"},"PeriodicalIF":3.3000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10880500/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell Adhesion & Migration","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/19336918.2024.2315629","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/2/20 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Copines are a family of calcium-dependent membrane-binding proteins. To study these proteins, anull mutant for cpnC was created in Dictyostelium, which has six copines genes (cpnA-cpnF). During development, cpnC- cells were able to aggregate, but did not form streams. Once aggregated into mounds, they formed large ring structures. cpnC- cells were less adherent to plastic substrates, but more adherent to other cells. These phenotypes correlated with changes in adhesion protein expression with decreased expression of SibA and increased expression of CsaA in developing cpnC- cells. We also measured the expression of RegA, a cAMP phosphodiesterase, and found that cpnC- cells have reduced RegA expression. The reduced RegA expression in cpnC- cells is most likely responsible for the observed phenotypes.
期刊介绍：
Cell Adhesion & Migration is a multi-disciplinary, peer reviewed open access journal that focuses on the biological or pathological implications of cell-cell and cell-microenvironment interactions. The main focus of this journal is fundamental science. The journal strives to serve a broad readership by regularly publishing review articles covering specific disciplines within the field, and by publishing focused issues that provide an overview on specific topics of interest within the field.
Cell Adhesion & Migration publishes relevant and timely original research, as well as authoritative overviews, commentaries, and perspectives, providing context for the work presented in Cell Adhesion & Migration and for key results published elsewhere. Original research papers may cover all topics important in the field of cell-cell and cell-matrix interactions. Cell Adhesion & Migration also publishes articles related to cell biomechanics, biomaterial, and development of related imaging technologies.
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