Natural variation of Grain size 3 allele differentially functions in regulating grain length in xian/indica and geng/japonica rice

IF 1.6 3区农林科学 Q2 AGRONOMY Euphytica Pub Date : 2024-02-27 DOI:10.1007/s10681-024-03293-7

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Abstract

Grain yield in rice is largely determined by grain size. Grain Size 3 (GS3) is a major quantitative trait locus for grain size. The C–A natural variation in the second exon of GS3 was reported to play an important role in regulating grain length in rice. Here we evaluate GS3 alleles among 303 germplasm accessions. The GS3^A allele was predominant in xian/indica (XI) accessions, whereas geng/japonica (GJ) accessions mainly carried GS3^C. The GS3 allele affected the grain length significantly in XI, while its function was minimal in GJ, indicating that introduction of GS3 alleles might be useful to modify grain length in XI breeding programs, but not in GJ breeding. The association between GS3 alleles and seed weight was not significant in any of the individual subpopulations, suggesting that the contribution of GS3 to grain weight could be slight in terms of different subspecies. To develop an effective marker for GS3, a penta-primer amplification-refractory mutation system (PARMS) marker exploiting a single-base mutation (C–A) was developed, which entailed lower cost and less time than other available markers, and should be useful for fine marker-assisted selection of grain length in XI accessions breeding.

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谷粒大小 3 等位基因的自然变异在调节籼稻和粳稻谷粒长度方面具有不同功能

摘要水稻的谷粒产量主要由谷粒大小决定。粒度 3（GS3）是粒度的一个主要数量性状基因座。据报道，GS3 第二外显子中的 C-A 自然变异在调节水稻谷粒长度方面起着重要作用。在此，我们对 303 份种质材料中的 GS3 等位基因进行了评估。GS3A等位基因在湘/籼（XI）品种中占优势，而耿/粳（GJ）品种主要携带GS3C。在 XI 中，GS3 等位基因对谷粒长度的影响很大，而在 GJ 中其作用很小，这表明在 XI 育种计划中引入 GS3 等位基因可能有助于改变谷粒长度，但在 GJ 育种中则无效。GS3等位基因与籽粒重量的关系在任何亚种中都不显著，这表明在不同亚种中，GS3对籽粒重量的贡献可能很小。为开发 GS3 的有效标记，开发了一种利用单碱基突变（C-A）的五元引物扩增-抑制突变系统（PARMS）标记，与其他可用标记相比，该标记成本低、时间短，可用于 XI 品种育种中谷粒长度的精细标记辅助选择。

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来源期刊

Euphytica 农林科学-农艺学

CiteScore

3.80

自引率

5.30%

发文量

157

审稿时长

4.5 months

期刊介绍： Euphytica is an international journal on theoretical and applied aspects of plant breeding. It publishes critical reviews and papers on the results of original research related to plant breeding. The integration of modern and traditional plant breeding is a growing field of research using transgenic crop plants and/or marker assisted breeding in combination with traditional breeding tools. The content should cover the interests of researchers directly or indirectly involved in plant breeding, at universities, breeding institutes, seed industries, plant biotech companies and industries using plant raw materials, and promote stability, adaptability and sustainability in agriculture and agro-industries.