QTL mapping and epistatic interactions for improved seed oil quality in safflower

IF 1.6 3区农林科学 Q2 AGRONOMY Euphytica Pub Date : 2024-09-10 DOI:10.1007/s10681-024-03408-0

Seyedeh Esmat Hashemi, Nafiseh Mahdi Nezhad, Ghasem Mohammadi-Nejad, Fatemeh Ebrahimi, Barat Ali Fakheri

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Abstract

Safflower oil with high oleic is an important source of oil due to its oxidative stability and nutritional value as well as its industrial benefits.

Identification of molecular markers associated with oil quality traits may aid in genotype selection in breeding efforts of safflower. To determine the genetic basis of seed oil, oleic acid, and linoleic acid content in safflower seeds, a recombinant inbred population was developed from a cross between Goldasht (low oleic line) and Mex 22–191 (high oleic line) by individual self-pollinated plants in each generation (from F2 to F9). Fatty acid components were detected by gas chromatography and subsequently analyzed by QTL analysis. The high variability in fatty acid composition was demonstrated and the first high-oleic safflower genotype was generated. A new linkage map with 69 AFLP and 45 ISSR polymorphic markers was made, covering 1472,441 cM of the safflower genome. A total of 63 individual QTLs and 238 epistatic QTLs, related to oil quality, were detected in 14 genomic regions. Some major QTLs with phenotypic variation greater than 10% were identified for the measured traits. These results demonstrate that major QTL may play an important role in increase the oleic acid of the genetic material used. Furthermore, the larger number of minor QTLs and epistatic QTLs, both of which have additive effects, indicates that the expression of these traits is also influenced by interactions between multiple genes. Our results provide additional genetic resources that can be used to precisely breed these traits in the future.

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红花种子油质改良的 QTL 图谱和表观相互作用

高油酸红花油因其氧化稳定性、营养价值和工业效益而成为重要的油料来源。鉴定与油质性状相关的分子标记有助于红花育种工作中的基因型选择。为了确定红花种子中籽油、油酸和亚油酸含量的遗传基础，研究人员从 Goldasht（低油酸品系）和 Mex 22-191（高油酸品系）杂交中培育了一个重组近交种群，每一代（从 F2 到 F9）都有单独的自花授粉植株。通过气相色谱法检测脂肪酸成分，然后进行 QTL 分析。结果表明脂肪酸成分的变异性很高，并产生了第一个高油酸红花基因型。利用 69 个 AFLP 和 45 个 ISSR 多态标记绘制了新的连接图，覆盖了红花基因组的 1472,441 cM。在 14 个基因组区域中，共检测到 63 个与油质有关的单个 QTL 和 238 个外显 QTL。在测定的性状中，发现了一些表型变异大于 10%的主要 QTL。这些结果表明，主要 QTL 在提高所用遗传物质的油酸含量方面可能起着重要作用。此外，较多的次要 QTL 和外显 QTL 都具有加性效应，这表明这些性状的表达还受到多个基因之间相互作用的影响。我们的研究结果提供了更多的遗传资源，可用于今后对这些性状进行精确育种。

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来源期刊

Euphytica 农林科学-农艺学

CiteScore

3.80

自引率

5.30%

发文量

157

审稿时长

4.5 months

期刊介绍： Euphytica is an international journal on theoretical and applied aspects of plant breeding. It publishes critical reviews and papers on the results of original research related to plant breeding. The integration of modern and traditional plant breeding is a growing field of research using transgenic crop plants and/or marker assisted breeding in combination with traditional breeding tools. The content should cover the interests of researchers directly or indirectly involved in plant breeding, at universities, breeding institutes, seed industries, plant biotech companies and industries using plant raw materials, and promote stability, adaptability and sustainability in agriculture and agro-industries.