A Fluorescent Sex-Sorting Technique for Insects with the Demonstration in Drosophila melanogaster.

IF 2 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY GEN biotechnology Pub Date : 2024-02-01 Epub Date: 2024-02-15 DOI:10.1089/genbio.2023.0041
Junru Liu, Danny Rayes, Omar S Akbari
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Abstract

Recent advances in insect genetic engineering offer alternative genetic biocontrol solutions to control populations of pests and disease vectors. While success has been achieved, sex-sorting remains problematic for scaling many genetic biocontrol interventions. Here, we describe the development of a genetically stable sex-sorting technique for female and male selection with a proof of concept in Drosophila melanogaster termed SEPARATOR (Sexing Element Produced by Alternative RNA-splicing of A Transgenic Observable Reporter). This elegant approach utilizes dominantly expressed fluorescent proteins and differentially spliced introns to ensure sex-specific expression. The system has the potential for adaptability to various insect species and application for high-throughput insect sex-sorting.

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以黑腹果蝇为示范的昆虫荧光性别分类技术。
昆虫基因工程的最新进展为控制害虫和疾病媒介种群提供了可供选择的基因生物控制解决方案。虽然已经取得了成功,但性别分选仍是许多基因生物控制干预措施推广的难题。在这里,我们介绍了一种用于雌性和雄性选择的遗传稳定的性别分类技术,并在黑腹果蝇中进行了概念验证,这种技术被称为 SEPARATOR(通过转基因可观察报告基因的 RNA 替代剪接产生的性别元素)。这种优雅的方法利用显性表达的荧光蛋白和差异剪接的内含子来确保性别特异性表达。该系统具有适应各种昆虫物种和应用于高通量昆虫性别分类的潜力。
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