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A Fluorescent Sex-Sorting Technique for Insects with the Demonstration in Drosophila melanogaster. 以黑腹果蝇为示范的昆虫荧光性别分类技术。
Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GEN biotechnology
Pub Date : 2024-02-01 Epub Date: 2024-02-15 DOI: 10.1089/genbio.2023.0041
Junru Liu, Danny Rayes, Omar S Akbari

Recent advances in insect genetic engineering offer alternative genetic biocontrol solutions to control populations of pests and disease vectors. While success has been achieved, sex-sorting remains problematic for scaling many genetic biocontrol interventions. Here, we describe the development of a genetically stable sex-sorting technique for female and male selection with a proof of concept in Drosophila melanogaster termed SEPARATOR (Sexing Element Produced by Alternative RNA-splicing of A Transgenic Observable Reporter). This elegant approach utilizes dominantly expressed fluorescent proteins and differentially spliced introns to ensure sex-specific expression. The system has the potential for adaptability to various insect species and application for high-throughput insect sex-sorting.

昆虫基因工程的最新进展为控制害虫和疾病媒介种群提供了可供选择的基因生物控制解决方案。虽然已经取得了成功，但性别分选仍是许多基因生物控制干预措施推广的难题。在这里，我们介绍了一种用于雌性和雄性选择的遗传稳定的性别分类技术，并在黑腹果蝇中进行了概念验证，这种技术被称为 SEPARATOR（通过转基因可观察报告基因的 RNA 替代剪接产生的性别元素）。这种优雅的方法利用显性表达的荧光蛋白和差异剪接的内含子来确保性别特异性表达。该系统具有适应各种昆虫物种和应用于高通量昆虫性别分类的潜力。
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引用次数: 0
The Physical View of the Origin of Genetic Engineering 基因工程起源的物理学观点
Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GEN biotechnology
Pub Date : 2023-12-07 DOI: 10.1089/genbio.2023.0037
Anna Yakimova, Denis Baranovskii, Sergei A. Ivanov, Peter Shegay, A. D. Kaprin, Ilya D. Klabukov
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引用次数: 0
In Vivo Prime Editing by Lipid Nanoparticle Co-Delivery of Chemically Modified pegRNA and Prime Editor mRNA 通过脂质纳米颗粒共同递送化学修饰的 pegRNA 和 Prime Editor mRNA 进行体内 Prime 编辑
Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GEN biotechnology
Pub Date : 2023-12-01 DOI: 10.1089/genbio.2023.0045
Zexiang Chen, Karen Kelly, Hao-Feng Cheng, Xiaolong Dong, Adam K. Hedger, Li Li, E. Sontheimer, Jonathan K. Watts
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引用次数: 0
Point and Click: DNA Polymerase Prime Editors Upgrade Gene Rewriting Arsenal 指点江山DNA 聚合酶主编辑器升级基因改写武器库
Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GEN biotechnology
Pub Date : 2023-12-01 DOI: 10.1089/genbio.2023.29120.jdg
Jonathan D. Grinstein
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引用次数: 0
Improved Cell-Type Identification and Comprehensive Mapping of Regulatory Features with Spatial Epigenomics 96-Channel Microfluidic Platform 利用空间表观组学 96 通道微流控平台改进细胞类型鉴定并全面绘制调控特征图
Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GEN biotechnology
Pub Date : 2023-12-01 DOI: 10.1089/genbio.2023.0044
Zev Kartiganer, Gumaro Rojas, Machele Riccio, Abigail Tyree, Katelyn Noronha, Molly Wetzel, Joshua Barnett, James McGann, Jennifer Garbarino, Daniel Massucci, Nouroddin Sotoudeh Chafi, Silas Decker, Arianna McDaniels, Jeffrey Sabina, David Levchenko, Jose Perez, Colin Ng, Kenneth Wang
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引用次数: 0
Defining the Rules of Biology in the Age of Genome Writing 在基因组写作时代定义生物学规则
Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GEN biotechnology
Pub Date : 2023-12-01 DOI: 10.1089/genbio.2023.29125.awo
A. Woolfson
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引用次数: 0
Drug Pricing Versus Precision Medicine 药物定价与精准医疗
Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GEN biotechnology
Pub Date : 2023-12-01 DOI: 10.1089/genbio.2023.29124.kda
Kevin Davies
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引用次数: 0
Revelry and Reminiscence: 50 Years of Recombinant DNA at Cold Spring Harbor Laboratory 狂欢与回忆：冷泉港实验室 DNA 重组 50 周年
Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GEN biotechnology
Pub Date : 2023-12-01 DOI: 10.1089/genbio.2023.29122.fli
Fay Lin
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引用次数: 0
Landmark CRISPR Approvals and the Rise of Epigenome Editing 具有里程碑意义的 CRISPR 批准和表观基因组编辑的兴起
Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GEN biotechnology
Pub Date : 2023-12-01 DOI: 10.1089/genbio.2023.29123.gli
G. Livshits
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引用次数: 0
AI, Bias, and Discrimination 人工智能、偏见和歧视
Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GEN biotechnology
Pub Date : 2023-12-01 DOI: 10.1089/genbio.2023.29126.editorial
Hana El-Samad
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引用次数: 0
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