Prevotella, a dominant bacterium in young people with stage Ⅲ periodontitis, related to the arachidonic acid metabolism pathway

IF 2.6 4区医学 Q3 IMMUNOLOGY Microbes and Infection Pub Date : 2024-05-01 DOI:10.1016/j.micinf.2024.105316

Qin Ye , Yaqiong Zhao , Jie Zhao , Zeyue Ouyang , Yao Feng , Jing Hu , Xiaolin Su , Ningxin Chen , Yun Chen , Li Tan , Yunzhi Feng , Yue Guo

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Abstract

Objects

As periodontitis progresses, the oral microbiome changes dynamically. The aim of this study is to evaluate the dominant bacteria of adults with stage III periodontitis and investigate potential pathways related to the dominant bacteria.

Materials and methods

16S rRNA sequencing was carried out to detect the differences in the oral microbiome between adult with stage Ⅰ and stage Ⅲ periodontitis and find the dominant bacteria in each group. The inhibitor of the predominant pathway for stage Ⅲ periodontitis was used to investigate the role of the dominant bacteria in periodontitis in vivo and in vitro.

Results

There was no significant difference in the α-diversity between the two groups. The results of β-diversity showed that the samples were divided into different groups according to the stage of periodontitis. The dominant bacteria in youths with stage Ⅲ periodontitis was Prevotella and may be related to the arachidonic acid metabolism pathway. Administration of SKF-86002 suppressed the expression of inflammation mediators in vivo and vitro.

Conclusions

Prevotella was the one dominant bacteria in young people with stage Ⅲ periodontitis and was related to the arachidonic acid metabolism pathway.

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与花生四烯酸代谢途径有关的Ⅲ期牙周炎年轻人中的优势菌--前牙菌。

对象：随着牙周炎的进展，口腔微生物组会发生动态变化。材料与方法：通过 16S rRNA 测序，检测Ⅰ期和Ⅲ期牙周炎成人口腔细菌的差异，并找出各组的优势菌。采用Ⅲ期牙周炎优势途径抑制剂研究优势菌在牙周炎体内外的作用：结果：两组α多样性无明显差异。β多样性结果显示，根据牙周炎的不同阶段，样本被分为不同的组别。在Ⅲ期牙周炎的青少年中，优势菌是普雷沃特氏菌，可能与花生四烯酸代谢途径有关。服用SKF-86002可抑制体内和体外炎症介质的表达：结论： Prevotella是年轻人Ⅲ期牙周炎的主要细菌，与花生四烯酸代谢途径有关。

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来源期刊

Microbes and Infection 医学-病毒学

CiteScore

12.60

自引率

1.70%

发文量

审稿时长

40 days

期刊介绍： Microbes and Infection publishes 10 peer-reviewed issues per year in all fields of infection and immunity, covering the different levels of host-microbe interactions, and in particular: the molecular biology and cell biology of the crosstalk between hosts (human and model organisms) and microbes (viruses, bacteria, parasites and fungi), including molecular virulence and evasion mechanisms. the immune response to infection, including pathogenesis and host susceptibility. emerging human infectious diseases. systems immunology. molecular epidemiology/genetics of host pathogen interactions. microbiota and host "interactions". vaccine development, including novel strategies and adjuvants. Clinical studies, accounts of clinical trials and biomarker studies in infectious diseases are within the scope of the journal. Microbes and Infection publishes articles on human pathogens or pathogens of model systems. However, articles on other microbes can be published if they contribute to our understanding of basic mechanisms of host-pathogen interactions. Purely descriptive and preliminary studies are discouraged.