Endophytic Bacillus amyloliquefaciens Mdgb15 is a potential biocontrol agent against tree peony gray mold caused by Botrytis cinerea

IF 1.7 3区农林科学 Q2 AGRONOMY European Journal of Plant Pathology Pub Date : 2024-03-04 DOI:10.1007/s10658-024-02838-9

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Abstract

Botrytis cinerea is the causal agent of gray mold disease affecting Paeoniaceae plants. The present study focuses on the potential of Bacillus amyloliquefaciens Mdgb15 as a promising biological control agent toward B. cinerea. The strain Mdgb15 showed the highest inhibition rate (55.56%) among all the strains tested against B. cinerea growth and was effective in controlling tree peony gray mold in detached leaves. A conventional PCR with three specific primer pairs detected lipopetides substance synthesis genes of Mdgb15. The results revealed that Mdgb15 had srfAA, fenA and ituD genes, which resulted in the production of surfactin, fengycin and iturin. An accurate qRT-PCR protocol with specifically designed primers was developed to characterize the expression of lipopeptide synthesis gene of Mdgb15. The qRT-PCR analysis revealed that the expression of the ituD and fenA genes in Mdgb15 was significantly up-regulated during the coculture with B. cinerea. Subsequently, the lipopeptides were extracted by acid precipitation of culture filtrate of Mdgb15 and were separated by Sephadex Gel chromatography. Three separated fractions exhibited strong antifungal activity against B. cinerea, and were identified as bacillomycin D by MALDI-TOF-MS analysis. This study has demonstrated that B. amyloliquefaciens Mdgb15 is a future potential new biocontrol agent for controlling tree peony gray mold disease. It suggests that the antifungal mechanisms of Mdgb15 may be at least partially associated with its ability to secret bacillomycin D.

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内生淀粉芽孢杆菌（Bacillus amyloliquefaciens）Mdgb15 是一种潜在的生物防治剂，可防治由灰霉病菌引起的牡丹灰霉病

摘要灰霉病（Botrytis cinerea）是影响芍药科植物的灰霉病的病原菌。本研究主要探讨了淀粉芽孢杆菌 Mdgb15 作为一种有前景的生物防治剂对灰霉病菌的潜力。在所有测试菌株中，Mdgb15 对灰霉病菌生长的抑制率最高（55.56%），并能有效控制牡丹脱落叶片上的灰霉病菌。使用三对特异引物进行常规 PCR，检测了 Mdgb15 的脂类物质合成基因。结果显示，Mdgb15 具有 srfAA、fenA 和 ituD 基因，可产生表面活性素、芬吉霉素和伊图灵。为了鉴定 Mdgb15 脂肽合成基因的表达情况，研究人员利用专门设计的引物制定了精确的 qRT-PCR 方案。qRT-PCR 分析表明，在与 B. cinerea 的共培养过程中，Mdgb15 中 ituD 和 fenA 基因的表达显著上调。随后，对 Mdgb15 的培养滤液进行酸沉淀提取脂肽，并用 Sephadex Gel 色谱进行分离。通过 MALDI-TOF-MS 分析，三个分离出来的馏分对银环蛇菌具有很强的抗真菌活性，并被鉴定为杆菌霉素 D。这项研究表明，淀粉芽孢杆菌 Mdgb15 是未来一种潜在的防治牡丹灰霉病的新型生物防治剂。研究表明，Mdgb15 的抗真菌机制可能至少部分与其分泌杆菌霉素 D 的能力有关。

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来源期刊

European Journal of Plant Pathology 农林科学-农艺学

CiteScore

4.20

自引率

5.60%

发文量

183

审稿时长

3 months

期刊介绍： The European Journal of Plant Pathology is an international journal publishing original articles in English dealing with fundamental and applied aspects of plant pathology; considering disease in agricultural and horticultural crops, forestry, and in natural plant populations. The types of articles published are :Original Research at the molecular, physiological, whole-plant and population levels; Mini-reviews on topics which are timely and of global rather than national or regional significance; Short Communications for important research findings that can be presented in an abbreviated format; and Letters-to-the-Editor, where these raise issues related to articles previously published in the journal. Submissions relating to disease vector biology and integrated crop protection are welcome. However, routine screenings of plant protection products, varietal trials for disease resistance, and biological control agents are not published in the journal unless framed in the context of strategic approaches to disease management.