Development of an Agrobacterium tumefaciens-mediate transformation system for somatic embryos and transcriptome analysis of LcMYB1’s inhibitory effect on somatic embryogenesis in Litchi chinensis.

IF 4.6 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Journal of Integrative Agriculture Pub Date : 2024-03-02 DOI:10.1016/j.jia.2024.03.007
Yaqi Qin, Bo Zhang, Xueliang Luo, Shiqian Wang, Jiaxin Fu, Zhike Zhang, Yonghua Qin, Jietang Zhao, Guibing Hu
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Abstract

Litchi holds paramount economic significance as a global fruit crop. However, the advancement of litchi functional genomics encounters substantial obstacles due to its recalcitrance to stable transformation. Here, we present an efficacious -mediated transformation system in somatic embryo of ‘Heiye’ litchi. This system was developed through meticulous optimization of variables encompassing explant selection, strain delineation, bacterium concentration, infection duration, and infection methodology. The subsequent validation of the transformation technique in litchi was realized through the ectopic expression of , resulting in the generation of transgenic calli. However, it was discerned that the differentiation of transgenic calli into somatic embryos encountered substantial challenges. To delve into the intricate molecular underpinnings of ’s inhibitory role in somatic embryo induction, a comprehensive transcriptome analysis was conducted encompassing embryogenic calli (C), globular embryos (G), and transgenic calli (TC). A total of 1166 common differentially expressed genes (DEGs) were identified between C-vs-G and C-vs-TC. Gene ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that these common DEGs were most related to plant hormone signal transduction pathways. Furthermore, RT-qPCR corroborated pronounced down-regulation of numerous genes intricately associated with somatic embryos induction within the transgenic calli. The development of this transformation system has provided valuable support for functional genomics research in litchi.
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开发农杆菌体细胞胚胎转化系统及 LcMYB1 对荔枝体细胞胚胎发生抑制作用的转录组分析。
荔枝作为一种全球水果作物,具有极其重要的经济意义。然而,由于荔枝不耐稳定转化,荔枝功能基因组学的发展遇到了巨大障碍。在此,我们提出了一种有效的荔枝 "黑叶 "体胚介导转化系统。该系统是通过对包括外植体选择、菌株划分、细菌浓度、感染持续时间和感染方法在内的变量进行细致的优化而开发出来的。随后,通过异位表达、产生转基因荔枝茧,在荔枝中实现了转化技术的验证。然而,转基因胼胝体分化为体细胞胚胎的过程遇到了巨大挑战。为了深入研究''在体细胞胚胎诱导过程中的抑制作用的复杂分子基础,我们对胚胎胼胝体(C)、球胚(G)和转基因胼胝体(TC)进行了全面的转录组分析。在 C-vs-G 和 C-vs-TC 之间共发现了 1166 个常见的差异表达基因(DEG)。基因本体(GO)注释和京都基因和基因组百科全书(KEGG)通路分析表明,这些常见的 DEGs 与植物激素信号转导通路的关系最为密切。此外,RT-qPCR 证实了转基因胼胝体中与体细胞胚胎诱导密切相关的许多基因明显下调。该转化系统的开发为荔枝功能基因组学研究提供了宝贵的支持。
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来源期刊
Journal of Integrative Agriculture
Journal of Integrative Agriculture AGRICULTURE, MULTIDISCIPLINARY-
CiteScore
7.90
自引率
4.20%
发文量
4817
审稿时长
3-6 weeks
期刊介绍: Journal of Integrative Agriculture publishes manuscripts in the categories of Commentary, Review, Research Article, Letter and Short Communication, focusing on the core subjects: Crop Genetics & Breeding, Germplasm Resources, Physiology, Biochemistry, Cultivation, Tillage, Plant Protection, Animal Science, Veterinary Science, Soil and Fertilization, Irrigation, Plant Nutrition, Agro-Environment & Ecology, Bio-material and Bio-energy, Food Science, Agricultural Economics and Management, Agricultural Information Science.
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