Pathogenic avian mycoplasmas show phenotypic differences in their biofilm forming ability compared to non-pathogenic species in vitro

IF 5.9 Q1 MICROBIOLOGY Biofilm Pub Date : 2024-03-06 DOI:10.1016/j.bioflm.2024.100190
Salvatore Catania , Marco Bottinelli , Alice Fincato , Annalucia Tondo , Andrea Matucci , Giorgia Nai , Verdiana Righetti , Francesco Abbate , Ana S. Ramírez , Federica Gobbo , Marianna Merenda
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Abstract

Mycoplasmas are known as the minimalist microorganisms in the microbes’ world. Their minimalist nature makes them highly sensitive to the environmental conditions and limits their ability to survive for extended periods outside their animal host. Nevertheless, there are documented instances of mycoplasma transmission over significant distances and this phenomenon may be linked to relatively unexplored abilities of mycoplasmas, such as their capacity to synthesize biofilm—the predominant mode of bacterial growth in nature. The authors decided to establish a method aimed at inducing the clustering of mycoplasma planktonic cells within a biofilm in vitro and subsequently assess the capacity of certain avian mycoplasmas to synthesize a biofilm. A total of 299 avian mycoplasma isolates were included in the study, encompassing both pathogenic (Mycoplasma gallisepticum, M. synoviae, M. meleagridis, M. iowae) and non-pathogenic species (M. gallinaceum, M. gallinarum, M. iners and M. pullorum). The authors successfully demonstrated the feasibility of inducing avian mycoplasmas to synthetize in vitro a biofilm, which can be visually quantified. The only species that did not produce any biofilm was M. iowae. In general, the pathogenic mycoplasmas produced greater quantities of biofilm compared to the non-pathogenic ones. Furthermore, it was observed that the ability to produce biofilm appeared to vary, both qualitatively and quantitatively, not only among different species but also among isolates of a single species. Future studies will be necessary to determine whether biofilm production plays a pivotal epidemiological role for the pathogenic avian mycoplasmas.

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与体外非致病性物种相比,致病性禽支原体的生物膜形成能力表现出表型差异
支原体被称为微生物世界中的最小微生物。它们的极简特性使其对环境条件高度敏感,并限制了它们在动物宿主之外长期存活的能力。尽管如此,支原体仍有远距离传播的记录,这种现象可能与支原体相对未被探索的能力有关,例如它们合成生物膜的能力--这是自然界细菌生长的主要模式。作者决定建立一种旨在诱导支原体浮游细胞在体外生物膜内聚集的方法,并随后评估某些禽类支原体合成生物膜的能力。这项研究共纳入了 299 个禽类支原体分离物,包括致病性支原体(Mycoplasma gallisepticum、M. synoviae、M. meleagridis、M. iowae)和非致病性支原体(M. gallinaceum、M. gallinarum、M. iners 和 M. pullorum)。作者成功证明了诱导禽类支原体在体外合成生物膜的可行性,这种生物膜可以用肉眼量化。唯一没有产生任何生物膜的是 M. iowae。一般来说,与非致病性支原体相比,致病性支原体产生的生物膜数量更多。此外,研究还发现,不仅不同种类的支原体之间,而且同一种类的不同分离物之间,产生生物膜的能力似乎在质和量两方面都存在差异。今后有必要开展研究,以确定生物膜的产生是否对致病性禽支原体的流行起着关键作用。
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来源期刊
Biofilm
Biofilm MICROBIOLOGY-
CiteScore
7.50
自引率
1.50%
发文量
30
审稿时长
57 days
期刊介绍:
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