Eucalypt seedlings lack a clear phosphate starvation response under low phosphorus availability

IF 2.2 4区生物学 Q2 PLANT SCIENCES Theoretical and Experimental Plant Physiology Pub Date : 2024-03-22 DOI:10.1007/s40626-024-00311-w

{"title":"Eucalypt seedlings lack a clear phosphate starvation response under low phosphorus availability","authors":"","doi":"10.1007/s40626-024-00311-w","DOIUrl":null,"url":null,"abstract":"<h3>Abstract</h3> In regions of low soil phosphorus (P) availability, such as many tropical and subtropical regions, the cultivation of eucalypts is common due to their adaptation to P-constrained soils. As in other trees, the molecular mechanisms underlying the phosphate starvation response (PSR) in eucalypts remain poorly understood. This study aimed to elucidate the molecular responses associated with PSR and assess the efficiency of P acquisition in five eucalypt species: Eucalyptus acmenoides, E. grandis, E. globulus, E. tereticornis, and Corymbia maculata. A greenhouse experiment was carried out in soil/substrate with low resin-extractable P (4.5 mg kg−1, Low P) and sufficient P (10.8 mg kg−1, Sufficient P) availability. After nine months growing in such conditions, various parameters were assessed, such as biomass production, P concentrations, P uptake efficiency (PUpE), and the expression of PSR-related genes. Overall, eucalypt plants exhibited a relatively weak response to low P availability, with slight variations in biomass production, P concentration, and PSR gene expression. C. maculata plants exhibited the highest PUpE under low P, while E. globulus exhibited the lowest. Among PSR-related genes, LPR1/2 in the roots of E. grandis, PDR2 in the roots of C. maculata, and phosphate transporters PHT1;6 and PHT1;8 in the roots of E. globulus, along with PHT1;12 in the roots of E. tereticornis, were induced under low P availability. Elevated PHT1 transcripts in the roots under sufficient P conditions, despite adequate leaf P concentrations, suggest potential interactions with other nutrient availability such as nitrogen, magnesium, and calcium, as well as symbiotic associations. Additionally, the upregulation SQD1 gene involved in membrane lipid remodeling in leaves of E. tereticornis, E. acmenoides, and C. maculata under low P suggests an improved P utilization efficiency. This study reveals the intricate and multifaceted nature of eucalypt responses to soil P availability. Despite the low P concentrations, eucalypt plants maintained foliar concentrations similar to those in the P-sufficient treatment suggesting a complex interplay of factors influencing PSR including nutrient balance.","PeriodicalId":23038,"journal":{"name":"Theoretical and Experimental Plant Physiology","volume":"2016 1","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Theoretical and Experimental Plant Physiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s40626-024-00311-w","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

In regions of low soil phosphorus (P) availability, such as many tropical and subtropical regions, the cultivation of eucalypts is common due to their adaptation to P-constrained soils. As in other trees, the molecular mechanisms underlying the phosphate starvation response (PSR) in eucalypts remain poorly understood. This study aimed to elucidate the molecular responses associated with PSR and assess the efficiency of P acquisition in five eucalypt species: Eucalyptus acmenoides, E. grandis, E. globulus, E. tereticornis, and Corymbia maculata. A greenhouse experiment was carried out in soil/substrate with low resin-extractable P (4.5 mg kg⁻¹, Low P) and sufficient P (10.8 mg kg⁻¹, Sufficient P) availability. After nine months growing in such conditions, various parameters were assessed, such as biomass production, P concentrations, P uptake efficiency (PUpE), and the expression of PSR-related genes. Overall, eucalypt plants exhibited a relatively weak response to low P availability, with slight variations in biomass production, P concentration, and PSR gene expression. C. maculata plants exhibited the highest PUpE under low P, while E. globulus exhibited the lowest. Among PSR-related genes, LPR1/2 in the roots of E. grandis, PDR2 in the roots of C. maculata, and phosphate transporters PHT1;6 and PHT1;8 in the roots of E. globulus, along with PHT1;12 in the roots of E. tereticornis, were induced under low P availability. Elevated PHT1 transcripts in the roots under sufficient P conditions, despite adequate leaf P concentrations, suggest potential interactions with other nutrient availability such as nitrogen, magnesium, and calcium, as well as symbiotic associations. Additionally, the upregulation SQD1 gene involved in membrane lipid remodeling in leaves of E. tereticornis, E. acmenoides, and C. maculata under low P suggests an improved P utilization efficiency. This study reveals the intricate and multifaceted nature of eucalypt responses to soil P availability. Despite the low P concentrations, eucalypt plants maintained foliar concentrations similar to those in the P-sufficient treatment suggesting a complex interplay of factors influencing PSR including nutrient balance.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

桉树幼苗在低磷供应条件下缺乏明显的磷酸盐饥饿反应

摘要在土壤磷（P）供应量较低的地区，如许多热带和亚热带地区，由于桉树对磷受限土壤的适应性，桉树的种植非常普遍。与其他树木一样，人们对桉树磷酸盐饥饿反应（PSR）的分子机制仍然知之甚少。本研究旨在阐明与磷酸盐饥饿反应相关的分子反应，并评估五种桉树获取磷酸盐的效率：E. globulus、E. tereticornis 和 Corymbia maculata。温室实验是在树脂可提取磷含量较低（4.5 毫克/千克，低磷）和磷含量充足（10.8 毫克/千克，充足磷）的土壤/基质中进行的。在这种条件下生长九个月后，对各种参数进行了评估，如生物量产量、钾浓度、钾吸收效率（PUpE）和 PSR 相关基因的表达。总体而言，桉树植物对低钾供应量的反应相对较弱，生物量产量、钾浓度和 PSR 基因表达量略有变化。在低钾条件下，C. maculata 植物表现出最高的 PUpE，而 E. globulus 表现出最低的 PUpE。在 PSR 相关基因中，大叶桉根部的 LPR1/2、大叶桉根部的 PDR2、球果桉根部的磷酸盐转运体 PHT1;6 和 PHT1;8，以及 E. tereticornis 根部的 PHT1;12，都在低 P 供应条件下被诱导。尽管叶片中的钾浓度充足，但在钾充足的条件下，根中的 PHT1 转录物升高，这表明它可能与氮、镁、钙等其他养分以及共生关系发生相互作用。此外，在低 P 条件下，E. tereticornis、E. acmenoides 和 C. maculata 叶片中参与膜脂重塑的 SQD1 基因上调，表明其对 P 的利用效率有所提高。这项研究揭示了桉树对土壤钾供应的复杂和多方面的反应。尽管钾的浓度很低，桉树植物叶片的钾浓度仍与钾充足的处理相似，这表明影响钾利用率的各种因素（包括养分平衡）之间存在着复杂的相互作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Theoretical and Experimental Plant Physiology PLANT SCIENCES-

CiteScore

4.20

自引率

7.70%

发文量

期刊介绍： The journal does not publish articles in taxonomy, anatomy, systematics and ecology unless they have a physiological approach related to the following sections: Biochemical Processes: primary and secondary metabolism, and biochemistry; Photobiology and Photosynthesis Processes; Cell Biology; Genes and Development; Plant Molecular Biology; Signaling and Response; Plant Nutrition; Growth and Differentiation: seed physiology, hormonal physiology and photomorphogenesis; Post-Harvest Physiology; Ecophysiology/Crop Physiology and Stress Physiology; Applied Plant Ecology; Plant-Microbe and Plant-Insect Interactions; Instrumentation in Plant Physiology; Education in Plant Physiology.