Reactive oxygen species in pollination drops of coniferous plants

IF 2.2 4区 生物学 Q2 PLANT SCIENCES Theoretical and Experimental Plant Physiology Pub Date : 2024-07-26 DOI:10.1007/s40626-024-00343-2
Maria Breygina, Oksana Luneva, Ksenia Babushkina, Olga Schekaleva, Svetlana Polevova
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Abstract

In flowering plants, reactive oxygen species (ROS) are actively involved in the regulation of sexual reproduction. Here we present the first evidence on the participation of ROS in sexual interactions in coniferous plants. In most gymnosperms, pollen hydrates and germinates in a pollination drop—a special type of ovular secretion. We studied the composition of this liquid for four conifer species from three families for the presence of ROS using the most sensitive method for detecting low concentrations of ROS—EPR (electron paramagnetic resonance) spectroscopy. ROS were present in pollination drops of all the plants studied, however, the composition of ROS was different: in Taxus, Pinus and Picea the main form was O2, in Thuja it was H2O2, reaching values comparable to stigma exudates of flowering plants. We tested the potential involvement of superoxide in the regulation of spruce pollen germination in vitro and found a strong stimulating effect on both total and bipolar germination efficiency. Thus, we found that conifers are characterized by the presence of ROS in the liquid intended for pollen germination, as well as in flowering plants. Hydrogen peroxide and superoxide radical, apparently represent a variety of ROS-based mechanisms for the regulation of pollen germination in vivo.

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针叶植物授粉滴中的活性氧
在开花植物中,活性氧(ROS)积极参与有性生殖的调控。在这里,我们首次提出了针叶植物中 ROS 参与有性互动的证据。在大多数裸子植物中,花粉在授粉滴--一种特殊的子房分泌物--中水合和发芽。我们使用检测低浓度 ROS 的最灵敏方法--EPR(电子顺磁共振)光谱法,研究了三个科的四个针叶树种的这种液体成分,以确定其中是否存在 ROS。所研究的所有植物的授粉液中都存在 ROS,但 ROS 的组成各不相同:Taxus、Pinus 和 Picea 的主要形式是 O⋅2-,Thuja 的主要形式是 H2O2,其值与开花植物的柱头渗出液相当。我们测试了超氧化物对云杉花粉体外萌发的潜在调节作用,发现超氧化物对总萌发效率和双极萌发效率都有很强的刺激作用。因此,我们发现针叶树和开花植物的花粉萌发液中都存在 ROS。过氧化氢和超氧自由基显然代表了多种基于 ROS 的体内花粉萌发调节机制。
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来源期刊
CiteScore
4.20
自引率
7.70%
发文量
32
期刊介绍: The journal does not publish articles in taxonomy, anatomy, systematics and ecology unless they have a physiological approach related to the following sections: Biochemical Processes: primary and secondary metabolism, and biochemistry; Photobiology and Photosynthesis Processes; Cell Biology; Genes and Development; Plant Molecular Biology; Signaling and Response; Plant Nutrition; Growth and Differentiation: seed physiology, hormonal physiology and photomorphogenesis; Post-Harvest Physiology; Ecophysiology/Crop Physiology and Stress Physiology; Applied Plant Ecology; Plant-Microbe and Plant-Insect Interactions; Instrumentation in Plant Physiology; Education in Plant Physiology.
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