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Reactive oxygen species in pollination drops of coniferous plants 针叶植物授粉滴中的活性氧
IF 2.6 4区 生物学 Q2 PLANT SCIENCES Pub Date : 2024-07-26 DOI: 10.1007/s40626-024-00343-2
Maria Breygina, Oksana Luneva, Ksenia Babushkina, Olga Schekaleva, Svetlana Polevova

In flowering plants, reactive oxygen species (ROS) are actively involved in the regulation of sexual reproduction. Here we present the first evidence on the participation of ROS in sexual interactions in coniferous plants. In most gymnosperms, pollen hydrates and germinates in a pollination drop—a special type of ovular secretion. We studied the composition of this liquid for four conifer species from three families for the presence of ROS using the most sensitive method for detecting low concentrations of ROS—EPR (electron paramagnetic resonance) spectroscopy. ROS were present in pollination drops of all the plants studied, however, the composition of ROS was different: in Taxus, Pinus and Picea the main form was O2, in Thuja it was H2O2, reaching values comparable to stigma exudates of flowering plants. We tested the potential involvement of superoxide in the regulation of spruce pollen germination in vitro and found a strong stimulating effect on both total and bipolar germination efficiency. Thus, we found that conifers are characterized by the presence of ROS in the liquid intended for pollen germination, as well as in flowering plants. Hydrogen peroxide and superoxide radical, apparently represent a variety of ROS-based mechanisms for the regulation of pollen germination in vivo.

在开花植物中,活性氧(ROS)积极参与有性生殖的调控。在这里,我们首次提出了针叶植物中 ROS 参与有性互动的证据。在大多数裸子植物中,花粉在授粉滴--一种特殊的子房分泌物--中水合和发芽。我们使用检测低浓度 ROS 的最灵敏方法--EPR(电子顺磁共振)光谱法,研究了三个科的四个针叶树种的这种液体成分,以确定其中是否存在 ROS。所研究的所有植物的授粉液中都存在 ROS,但 ROS 的组成各不相同:Taxus、Pinus 和 Picea 的主要形式是 O⋅2-,Thuja 的主要形式是 H2O2,其值与开花植物的柱头渗出液相当。我们测试了超氧化物对云杉花粉体外萌发的潜在调节作用,发现超氧化物对总萌发效率和双极萌发效率都有很强的刺激作用。因此,我们发现针叶树和开花植物的花粉萌发液中都存在 ROS。过氧化氢和超氧自由基显然代表了多种基于 ROS 的体内花粉萌发调节机制。
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引用次数: 0
Ammonium induces aquaporin gene expression in Guzmania monostachia (Bromeliaceae) under drought 干旱条件下铵盐诱导古马菌(Bromeliaceae)水蒸素基因的表达
IF 2.6 4区 生物学 Q2 PLANT SCIENCES Pub Date : 2024-07-24 DOI: 10.1007/s40626-024-00344-1
Victória Carvalho, Paula Natália Pereira, Bruno Nobuya Katayama Gobara, Marília Gaspar, Helenice Mercier

The leaves of Guzmania monostachia, a tank epiphytic bromeliad, present a functional division: the base performs water and nutrient uptake through absorbing trichomes, while the apex presents higher photosynthetic activity and nitrogen (N) assimilation. Drought conditions induce Crassulacean acid metabolism (CAM) in the apex portion, with N nutrition influencing this process. Ammonium (NH4+) induces higher CAM activity, reducing greater water loss compared to nitrate (NO3). Aquaporins play a crucial role in regulating membrane permeability in leaf tissues under drought. This study aimed to evaluate how different N sources (NO3 and NH4+) in combination with drought affect the diurnal gene expression of aquaporins along the leaf blade of G. monostachia. Detached leaves were immersed in 30% (w/v) polyethylene glycol 6000 solution containing NO3 or NH4+ for 7 days. Leaf apexes and bases were harvested at dawn and dusk. At dawn, NH4+ improved water conservation and upregulated the expression of five aquaporin genes in the apex compared to NO3, including GmNIP5;1. In the leaf base at dawn, GmNIP5;1 was the most upregulated gene by NH4+, suggesting its involvement in NH4+ transport to the apex, intensifying CAM and other aquaporin genes' expression under drought. At dusk, NH4+ led to pronounced downregulation of TIPs and NIPs than NO3 in both leaf portions, possibly ensuring lower membrane permeability and higher water retention during drought. These results offer valuable insights for future studies on the functions of aquaporins in drought tolerance and nitrogen-use efficiency in plants.

水箱附生凤梨属植物 Guzmania monostachia 的叶片有功能分区:基部通过吸水毛状体吸收水分和养分,而先端则具有较高的光合作用活性和氮(N)同化作用。干旱条件会诱导顶端部分的茎基酸代谢(CAM),氮营养会影响这一过程。与硝酸盐(NO3-)相比,铵(NH4+)诱导更高的 CAM 活性,减少更多的水分流失。在干旱条件下,水汽素在调节叶片组织的膜渗透性方面起着至关重要的作用。本研究旨在评估不同氮源(NO3- 和 NH4+)与干旱的结合如何影响单子叶植物叶片上水汽素的昼夜基因表达。将分离的叶片浸泡在含 NO3- 或 NH4+ 的 30% (w/v)聚乙二醇 6000 溶液中 7 天。在黎明和黄昏收获叶片的先端和基部。与 NO3- 相比,黎明时 NH4+ 提高了叶片的保水性,并上调了叶片先端五个水蒸气素基因的表达,其中包括 GmNIP5;1。在黎明时的叶片基部,GmNIP5;1 是受 NH4+ 上调最多的基因,这表明它参与了 NH4+ 向先端的运输,从而加强了 CAM 和其他水蒸气素基因在干旱条件下的表达。黄昏时分,NH4+导致两片叶片中的TIPs和NIPs比NO3-明显下调,这可能确保了干旱时较低的膜通透性和较高的保水性。这些结果为今后研究水蒸发蛋白在植物耐旱性和氮利用效率中的功能提供了宝贵的启示。
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引用次数: 0
The nematophagous root endophyte Pochonia chlamydosporia enhances tolerance to drought in soybean 嗜线虫根内生菌 Pochonia chlamydosporia 能增强大豆对干旱的耐受性
IF 2.6 4区 生物学 Q2 PLANT SCIENCES Pub Date : 2024-07-09 DOI: 10.1007/s40626-024-00341-4
Juliano Mendonça Rodrigues, Analu Zanotti Ávila, Angélica de Souza Gouveia, Ian de Paula Alves Pinto, Monique da Silva Bonjour, Renata Maria Strozi Alves Meira, Elizabeth Pacheco Batista Fontes, Leandro Grassi de Freitas, Maria Goreti de Almeida Oliveira, Humberto Josué de Oliveira Ramos

Background

Climate changes have led to fluctuations in rainfall, reducing crop productivity during drought conditions. In addition to developing tolerant genotypes, plant growth-promoting microorganisms could be applied to maintain sustainable agriculture under environmental stresses.

Objective

Therefore, in this study the ability of the nematophagous fungus P. chlamydosporia to promote drought tolerance in soybean plants was assessed.

Results

Under water deficit conditions, the inoculated plants exhibited a one-day delay in reaching the same water potential as the noninoculated plants. Fungal colonization improved physiological parameters associated with drought tolerance, resulting in a 2- to 5-fold increase in water use efficiency (A/E). Moreover, the behavior of the inoculated drought-sensitive BR16 plants resembled that of the drought-tolerant parental Embrapa 48. The relative water content (RWC) increased from 15% to 26% in the leaves and roots of inoculated plants of both genotypes under water deficit. Consequently, the intrinsic efficiency of water use (A/gs) was 6.7-fold greater in the inoculated BR 16 plants under water deficit. The increase in drought tolerance may be related to the induction of root growth in inoculated plants, although this increase was not associated with hydraulic conductivity. Similarly, fungal inoculation led to increased concentrations of phenolics, which directly or indirectly contributed to drought tolerance. Reduced concentrations of proline and ABA confirmed that P. chlamydosporia alleviated water deficit stress. Conversely, the increases in spermine and spermidine concentrations may act as osmoprotectants, relieving the water deficit in the roots.

Conclusion

Thus, P. chlamydosporia inoculation may contribute to maintaining soybean productivity during periods of limited water availability.

背景气候变化导致降雨量波动,降低了干旱条件下的作物产量。因此,本研究评估了嗜线虫真菌 P. chlamydosporia 促进大豆植株耐旱性的能力。结果在缺水条件下,接种植株在达到与未接种植株相同的水势方面表现出一天的延迟。真菌定殖改善了与耐旱性相关的生理参数,使水分利用效率(A/E)提高了 2 到 5 倍。此外,接种后对干旱敏感的 BR16 植物的表现与耐旱亲本 Embrapa 48 相似。在缺水条件下,两种基因型的接种植株叶片和根部的相对含水量(RWC)从 15%增加到 26%。因此,在缺水情况下,接种 BR 16 植物的内在水分利用效率(A/gs)是原来的 6.7 倍。耐旱性的提高可能与诱导接种植株的根系生长有关,尽管这种提高与水导率无关。同样,真菌接种导致酚类物质浓度增加,直接或间接地提高了耐旱性。脯氨酸和 ABA 浓度的降低证实衣壳菌减轻了缺水胁迫。相反,精胺和亚精胺浓度的增加可能起到渗透保护剂的作用,缓解了根部的缺水状况。
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引用次数: 0
The expression of 1-Cys-PRX reflects changes in Coffea arabica seed quality during storage 1-Cys-PRX 的表达反映了阿拉伯咖啡种子在贮藏期间的质量变化
IF 2.6 4区 生物学 Q2 PLANT SCIENCES Pub Date : 2024-06-25 DOI: 10.1007/s40626-024-00336-1
Thamires Lopes Ribeiro, Édila Vilela de Resende Von Pinho, Elise de Matos Pereira, Heloisa Oliveira dos Santos, Wilson Vicente Souza Pereira

Understanding the physiological aspects associated with the response of Coffea arabica seeds to drying is crucial for enhancing their production. We assessed the physiological quality and expression of the 1-Cys-PRX protein in coffee seeds subjected to drying and storage. Seeds of Coffea arabica L. were dried to water contents of 40, 20, and 10% and stored for 60, 120, and 180 days. The physiological quality of the seeds was evaluated through germination, emergence percentage, and speed index (IVE) tests. The expression of the 1-Cys-PRX protein was also analyzed using Western blotting. Greater PRX protein expression was detected in the coffee seeds dried with 20 and 10% water throughout storage, while no protein expression was detected in the seeds with 46% water (control treatment) or in the seeds with 40% water after 60 days of storage. The physiological quality of Coffea arabica seeds is influenced by water content and the storage period. A greater expression of peroxiredoxin protein was detected in coffee seeds dried with 20% and 10% water. There was a reduction in seed vigor when the plants were dry, particularly at 20% and 10% water contents.

了解与阿拉伯咖啡豆种子对干燥的反应有关的生理方面对提高其产量至关重要。我们评估了经过干燥和储藏的咖啡种子的生理质量和 1-Cys-PRX 蛋白的表达。将阿拉伯咖啡(Coffea arabica L.)种子干燥至含水量为 40%、20% 和 10%,并分别储存 60 天、120 天和 180 天。通过发芽率、萌发率和速度指数(IVE)测试评估了种子的生理质量。此外,还使用 Western 印迹法分析了 1-Cys-PRX 蛋白的表达情况。在整个贮藏过程中,用 20%和 10%的水烘干的咖啡种子中检测到较多的 PRX 蛋白表达,而在贮藏 60 天后,用 46% 的水(对照处理)或用 40% 的水烘干的种子中未检测到蛋白表达。阿拉伯咖啡种子的生理质量受含水量和储藏期的影响。在用 20% 和 10% 水烘干的咖啡种子中,过氧化物酶蛋白的表达量更大。当植株干燥时,种子活力会降低,尤其是在含水量为 20% 和 10% 的情况下。
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引用次数: 0
Inhibition of polyamine homeostasis facilitates root extension by modulating IAA and PIN1 distribution in etiolated salt-stressed sunflower seedlings 抑制多胺平衡可通过调节盐胁迫向日葵幼苗中 IAA 和 PIN1 的分布促进根系伸展
IF 2.6 4区 生物学 Q2 PLANT SCIENCES Pub Date : 2024-06-24 DOI: 10.1007/s40626-024-00342-3
Aditi Tailor, Satish C. Bhatla

Plant root growth and development undergo significant alterations as an adaptation to environmental stressful conditions. Remodeling of roots exposed to salinity is coordinated by complex interactions among various signaling pathways involving phytohormones, nitric oxide (NO), and reactive oxygen species (ROS). Polyamines (PAs), small, cationic amine molecules with diverse roles in plant growth and stress responses, are also known to influence root morphology. Studies reported that treatment of sunflower seedlings with PA biosynthesis inhibitors [DFMA (DL-α-difluoromethylarginine) or DFMO (DL-α-difluoromethylornithine)], promotes extension growth of primary roots both in the absence or presence of NaCl. This work explores the possible role of PAs and their crosstalk with auxin signaling in the modeling of the root morphology in etiolated sunflower seedlings. We observed that inhibition of root growth by salinity is possibly governed by a disruption of the polar localization of PIN1 (auxin efflux protein) leading to IAA deficiency in the root apex. Application of PA inhibitors (DFMA or DFMO), in contrast, brings about an enhancement in IAA accumulation in the root apices of seedlings relative to control (−NaCl), albeit to a lesser degree in seedlings also exposed simultaneously to 120 mM NaCl. These alterations in IAA accumulation coincide with changes in primary root extension previously reported in sunflower seedlings in response to treatment with PA inhibitors, both in the absence or presence of NaCl. We found that the enhancement in root extension observed in seedlings subjected to a combined treatment of PA biosynthesis inhibitors and NaCl possibly involves the maintenance of polar distribution of PIN1 in the root cells which, in turn, may be responsible for the restoration of IAA distribution in the root apex to further support the observed extension growth of primary root. On the other hand, the role of nitric oxide and hydrogen peroxide in the observed PA inhibitor-triggered response on root extension remains uncertain at present. Therefore, a possible role of PAs and their crosstalk with auxin is evident in root architecture remodeling in sunflower seedlings exposed to salinity.

植物根系的生长和发育会发生重大变化,以适应环境胁迫条件。暴露于盐度的根的重塑是由涉及植物激素、一氧化氮(NO)和活性氧(ROS)的各种信号通路之间复杂的相互作用协调的。多胺(PAs)是一种阳离子小胺分子,在植物生长和胁迫反应中具有多种作用,也被认为会影响根的形态。研究表明,用 PA 生物合成抑制剂[DFMA(DL-α-二氟甲基精氨酸)或 DFMO(DL-α-二氟甲基鸟氨酸)]处理向日葵幼苗,可促进主根在无 NaCl 或有 NaCl 的情况下伸展生长。本研究探讨了 PAs 及其与辅助素信号传导在向日葵幼苗根系形态建模中可能发挥的作用。我们观察到,盐度对根生长的抑制可能是由 PIN1(辅素外流蛋白)极性定位的破坏导致的根顶端 IAA 缺乏。与此相反,施用 PA 抑制剂(DFMA 或 DFMO)后,相对于对照组(-NaCl),幼苗根尖的 IAA 积累增加,尽管同时暴露于 120 毫摩尔 NaCl 的幼苗增加的程度较小。IAA 积累的这些变化与之前报道的向日葵幼苗在无 NaCl 或有 NaCl 的情况下对 PA 抑制剂处理的主根伸展变化相吻合。我们发现,在接受 PA 生物合成抑制剂和 NaCl 联合处理的幼苗中观察到的根系伸展的增强可能涉及 PIN1 在根细胞中极性分布的维持,这反过来又可能是恢复 IAA 在根顶分布的原因,从而进一步支持观察到的主根的伸展生长。另一方面,一氧化氮和过氧化氢在所观察到的 PA 抑制剂触发的根延伸反应中的作用目前仍不确定。因此,在向日葵幼苗暴露于盐度条件下的根系结构重塑过程中,PAs 及其与 auxin 之间的串扰作用是显而易见的。
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引用次数: 0
Drought induces DNA methylation balance‐related genes in a stage-dependent manner during tomato fruit ripening 在番茄果实成熟过程中,干旱以阶段依赖性方式诱导 DNA 甲基化平衡相关基因
IF 2.6 4区 生物学 Q2 PLANT SCIENCES Pub Date : 2024-06-21 DOI: 10.1007/s40626-024-00339-y
Paulo H. G. A. de Oliveira, Ana C. O. Barbosa, Nathiele S. Araújo, Marcio G. C. Costa

DNA methylation balance plays an important regulatory role in drought response and tolerance and also in the control of tomato fruit ripening, and its study may help to understand how fleshy fruits respond to drought. This work aimed to investigate how drought affects the expression profile of genes related to DNA methylation balance during tomato fruit ripening. Micro-Tom tomato plants were subjected to irrigated control (C) and two water deficit intensities (T1 and T2) treatments and analyzed for leaf gas exchange rates, leaf water potential (Ψw) and expression of genes encoding DNA methylases/demethylases during the mature green (MG), breaker (BR) and red ripe (RR) stages of fruit ripening. Water deficit promoted a significant reduction in the values of Ψw, photosynthetic rate (A), transpiration (E), stomatal conductance (gs), regardless of its applied intensity. In silico analysis of RNA-Seq data showed that CHROMOMETHYLASE 2 (SlCMT2) and DEMETER-LIKE 2 (SlDML2) were those, among all tomato DNA methylases/demethylases genes, that showed dramatic and opposite changes in expression during fruit ripening. Water deficit positively regulated the expression of SlCMT2 in the MG stage and SlDML2 in BR, with no significant variations in expression for these genes in the other stages of fruit ripening. These results show that genes related to DNA methylation balance have their expression further increased by water deficit in the fruit ripening stages in which they act, suggesting that drought enhances, but does not disrupt, the intrinsically programmed DNA methylation dynamics necessary for ripening progression.

DNA 甲基化平衡在干旱响应和耐旱性以及番茄果实成熟调控中起着重要的调控作用,对其研究可能有助于了解肉质果实如何响应干旱。本研究旨在探讨干旱如何影响番茄果实成熟过程中 DNA 甲基化平衡相关基因的表达谱。将微型番茄植株置于灌溉对照(C)和两种水分亏缺强度(T1和T2)处理下,分析了果实成熟期(MG)、破碎期(BR)和红熟期(RR)的叶片气体交换速率、叶片水势(Ψw)和编码DNA甲基化酶/脱甲基酶基因的表达。水分亏缺导致Ψw、光合速率(A)、蒸腾(E)、气孔导度(gs)的值明显下降,而与施加的强度无关。对 RNA-Seq 数据的硅学分析表明,在所有番茄 DNA 甲基化酶/脱甲基酶基因中,CHROMOMETHYLASE 2(SlCMT2)和 DEMETER-LIKE 2(SlDML2)在果实成熟过程中的表达量出现了巨大的相反变化。水分亏缺正向调节 MG 阶段 SlCMT2 和 BR 阶段 SlDML2 的表达,而在果实成熟的其他阶段,这些基因的表达没有显著变化。这些结果表明,与 DNA 甲基化平衡有关的基因在其作用的果实成熟阶段会因缺水而进一步提高表达量,这表明干旱会增强但不会破坏成熟过程中所需的内在程序化 DNA 甲基化动态。
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引用次数: 0
Why not be random to build a woody crown network? 为什么不随机建立树冠网络?
IF 2.6 4区 生物学 Q2 PLANT SCIENCES Pub Date : 2024-05-27 DOI: 10.1007/s40626-024-00340-5
Carlos Henrique Britto de Assis Prado

We compared the decomposition, topology, and properties of 58 natural woody crown networks (WCN) from Cerrado and Caatinga vegetation with the same number of WCN built randomly at different sizes, from 10 to 248 nodes (the branching regions). The random distribution of nodes did not wrack the intrinsic relationships between WCN properties and created woody crowns similar to those found in Caatinga with relatively safe hydraulics to face severe water and other stresses. Nevertheless, growing by random node addition is an inflexible, unintentional procedure incapable of creating WCN-like Cerrado trees. These Cerrado trees showed WCN with less laterality and basitony, potentially achieving higher maximum crown height, but inherently had a riskier hydraulic architecture with lower navigability between nodes. Hence, random node distribution is unsuitable for building WCN in environments with mild stresses where trees typically attain considerable height. A sense of node numerosity (abundance), location of node insertion (rectilinear or lateral), node relative position (near or far from the initial node) along the woody axis, and anticipation behavior are essential to construct a suitable and adaptable WCN in each changing environment. Besides remarkable structural plasticity, the cognition-related features could be accomplished by some constitutional random traits of WCN, constructing safe hydraulic architecture even by haphazard node addition, and modulating the WCN decomposition and topology within limits through a blueprint project.

我们比较了赛拉多(Cerrado)和卡廷加(Caatinga)植被中 58 个天然树冠网络(WCN)的分解、拓扑结构和特性,以及随机建立的相同数量、不同大小(从 10 节点到 248 节点)的树冠网络(分支区域)。节点的随机分布并没有破坏 WCN 特性之间的内在联系,而且所形成的树冠与 Caatinga 中的树冠相似,其水力相对安全,可应对严重的水压力和其他压力。然而,随机添加节点的生长方式缺乏灵活性,无法创造出类似于WCN的Cerrado树木。这些 Cerrado 树表现出的 WCN 侧向性和基部性较小,有可能达到更高的最大冠高,但其固有的水力结构风险较大,节点之间的通航能力较低。因此,在树木通常能达到相当高度的温和压力环境中,随机节点分布不适合建立 WCN。对节点数量(丰度)、节点插入位置(直线或横向)、节点沿木质轴的相对位置(离初始节点近或远)以及预期行为的感知,对于在各种变化的环境中构建合适且适应性强的 WCN 至关重要。除了显著的结构可塑性外,与认知相关的特征还可以通过WCN的一些宪法随机特征来实现,甚至通过胡乱增加节点来构建安全的水力结构,以及通过蓝图工程在一定范围内调节WCN的分解和拓扑结构。
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引用次数: 0
Plant-life vocabulary as metaphors for post-normal planetary challenges 以植物生命词汇隐喻正常状态后地球面临的挑战
IF 2.6 4区 生物学 Q2 PLANT SCIENCES Pub Date : 2024-05-13 DOI: 10.1007/s40626-024-00333-4
F. R. Scarano, A. C. F. Aguiar, V. L. Holz, A. L. de Macedo, A. Lombardi, L. S. dos Santos, F. Koch

The ongoing post-normal times face two intertwining imperatives: sustainability and digital technology. Interestingly, the languages that address these imperatives are increasingly making use of concepts historically associated to or confined to the biological sciences, in particular botany and plant physiology. We address this phenomenon by applying two distinct but interacting conceptual frameworks. First, that of language ecology, i.e., the study of interactions between any given language and its environment. Language has an anticipatory nature, since it is an essential part of how we organise memory and past experience, therefore helping us to understand the present and to project the future. This paper surveys five biological concepts: autopoiesis, ecosystem, exaptation, regeneration, seed. By using an academic search engine, we assessed how often they have been used in academic publications related to biology/botany as compared to other fields. We found that their meanings are being expanded by their use in the sustainability and/or digital technology realms. Motivations for these uses include biomimetics, biophilia and/or, more simply, manipulation of words with the intention to create specific public or private rhetoric. Second, we examined this linguistic pattern as a symptom of ontological expansion, i.e., the integration and dialogue between technical innovation, philosophy and biology creating space for the emergence of new domains of experience and meanings that did not exist before. This research indicates that botany—and plant physiology in particular—can be an important source of inspiration for futures studies.

正在到来的后正常时代面临着两个相互交织的当务之急:可持续性和数字技术。有趣的是,解决这些当务之急的语言越来越多地使用历史上与生物科学相关或仅限于生物科学的概念,特别是植物学和植物生理学。我们通过应用两个不同但相互影响的概念框架来解决这一现象。首先是语言生态学,即研究任何特定语言与其环境之间的相互作用。语言具有预测性,因为它是我们组织记忆和过去经验的重要组成部分,因此有助于我们理解现在和预测未来。本文探讨了五个生物学概念:自生、生态系统、外适应、再生、种子。通过使用学术搜索引擎,我们评估了与其他领域相比,这些概念在与生物学/植物学相关的学术出版物中的使用频率。我们发现,它们在可持续发展和/或数字技术领域的使用拓展了它们的含义。这些使用的动机包括生物仿生学、生物爱好者和/或,更简单地说,为了创造特定的公共或私人修辞而操纵词语。其次,我们将这种语言模式视为本体论扩展的一种表征,即技术创新、哲学和生物学之间的融合与对话为以前不存在的新的经验和意义领域的出现创造了空间。这项研究表明,植物学--尤其是植物生理学--可以成为未来研究的重要灵感来源。
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引用次数: 0
Positive modulation of selenium on photosynthetic performance in soybean under iron depletion 硒对缺铁条件下大豆光合作用性能的正向调节作用
IF 2.6 4区 生物学 Q2 PLANT SCIENCES Pub Date : 2024-05-07 DOI: 10.1007/s40626-024-00330-7
Allan de Marcos Lapaz, Camila Hatsu Pereira Yoshida, Daniel Gomes Coelho, Wagner Luiz Araujo, Maximiller Dal-Bianco, Cleberson Ribeiro

Although iron (Fe) is abundant in most agricultural soils, its bioavailability to plants is limited. The Fe deficiency can cause significant changes in plant metabolites, impacting the plant’s life cycle. In this context, selenium (Se) has been shown promising effects against Fe deficiency. However, there are few studies addressing the role of Se against the deleterious effects of Fe deficiency, mainly with the soybean crop. Hence, this study aimed to evaluate the effects of Se on dry mass, Fe concentration in the roots and shoots, as well as the photosynthetic performance and primary metabolism in soybean plants subjected to Fe deficiency. The experimental design used was completely randomized with 4 treatments: 1) absence of Fe without Se; 2) absence of Fe with Se; 3) sufficiency of Fe without Se (control); and 4) sufficiency of Fe with Se. Our results demonstrated that Fe deficiency combined with the absence of Se significantly reduced shoot and root dry mass, as well as Fe concentration in plants. Additionally, Fe deficiency had a detrimental impact on photosynthetic traits, whereas Se-treated plants exhibited a higher net CO2 assimilation rate and improved carboxylation efficiency and photochemical characteristics. Moreover, Fe deficiency negatively influenced primary metabolism, leading to the altered accumulation of sucrose and amino acids, and reduced protein concentration and ammonia. In contrast, Se-treated plants showed lower accumulation of sucrose and maintenance of protein concentration. These findings highlight the potential of Se as a valuable intervention to mitigate Fe deficiency in soybean crops.

虽然铁(Fe)在大多数农业土壤中含量丰富,但其对植物的生物利用率有限。缺铁会导致植物代谢产物发生重大变化,影响植物的生命周期。在这种情况下,硒(Se)被证明对防止缺铁有很好的效果。然而,很少有研究探讨硒对铁缺乏有害影响的作用,主要是对大豆作物的影响。因此,本研究旨在评估 Se 对缺铁大豆植株的干重、根和芽中铁的浓度以及光合作用和初级代谢的影响。实验采用完全随机设计,共设 4 个处理:1)缺铁不加硒;2)缺铁加硒;3)缺铁加硒(对照);4)缺铁加硒。我们的研究结果表明,缺铁加缺硒会显著降低植物的芽和根的干重以及铁的浓度。此外,缺铁还会对光合特性产生不利影响,而经过硒处理的植株则表现出更高的二氧化碳净同化率,并提高了羧化效率和光化学特性。此外,缺铁还对初级代谢产生负面影响,导致蔗糖和氨基酸的积累发生变化,蛋白质浓度和氨含量降低。相比之下,Se 处理的植株蔗糖积累较少,蛋白质浓度保持不变。这些发现凸显了 Se 作为一种有价值的干预措施来缓解大豆作物中铁缺乏症的潜力。
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引用次数: 0
The core stomatal proteins SPCH, MUTE and FAMA associate with both types of ICE proteins, VrICE1 and VrICE4, in Vitis riparia 葡萄树的核心气孔蛋白 SPCH、MUTE 和 FAMA 与两种类型的 ICE 蛋白(VrICE1 和 VrICE4)都有关联
IF 2.6 4区 生物学 Q2 PLANT SCIENCES Pub Date : 2024-05-04 DOI: 10.1007/s40626-024-00331-6
M. Atikur Rahman, Alison Edge, Layla Alibabai, Annette Nassuth

SPEECHLESS (SPCH), MUTE and FAMA drive stomatal development in Arabidopsis. They do so in association with SCREAM/INDUCER OF CBF EXPRESSION (SCRM/ICE) proteins. Orthologous proteins have also been reported for other plants, including now for Vitis, suggesting that a similar process occurs during stomata formation also in other plants. However, the details of this process likely vary because the Vitis protein sequences are most similar to those of dicots other than Arabidopsis. We recently reported a 2nd type of ICE protein in angiosperms, VrICE4L, with unique sequences in its putative protein-interacting bHLH and ACTL domains. This brings up the question whether this second ICE protein is also involved in stomatal formation. Overexpression of any one of the Vitis riparia SPCH, MUTE, FAMA or ICE proteins was found to affect stomata numbers in tobacco leaves. Localization, bimolecular fluorescence complementation (BiFC) and pull-down experiments pointed that VrSPCH, VrMUTE and VrFAMA can interact with both types of ICE proteins present in angiosperms, represented by VrICE1 and VrICE4. Taken together, these findings suggest that stomatal development in Vitis involves SPCH/ICE, MUTE/ICE and FAMA/ICE complexes with functions that are less similar to those in Arabidopsis than to those in other dicots.

SPEECHLESS(SPCH)、MUTE 和 FAMA 驱动拟南芥的气孔发育。它们与 SCREAM/INDUCER OF CBF EXPRESSION(SCRM/ICE)蛋白共同发挥作用。其他植物的同源蛋白也有报道,包括现在的葡萄属植物,这表明在其他植物的气孔形成过程中也有类似的过程。然而,这一过程的细节可能有所不同,因为葡萄属植物的蛋白质序列与拟南芥以外的双子叶植物的蛋白质序列最为相似。我们最近报告了被子植物中的第二种 ICE 蛋白--VrICE4L,其推测的蛋白相互作用 bHLH 和 ACTL 结构域具有独特的序列。这就提出了第二个 ICE 蛋白是否也参与气孔形成的问题。研究发现,过量表达任何一种葡萄孢 SPCH、MUTE、FAMA 或 ICE 蛋白都会影响烟草叶片的气孔数量。定位、双分子荧光互补(BiFC)和牵引实验表明,VrSPCH、VrMUTE 和 VrFAMA 可以与被子植物中存在的两种 ICE 蛋白(以 VrICE1 和 VrICE4 为代表)相互作用。综上所述,这些研究结果表明,葡萄气孔发育涉及 SPCH/ICE、MUTE/ICE 和 FAMA/ICE 复合物,其功能与拟南芥的相似性低于其他双子叶植物。
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引用次数: 0
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Theoretical and Experimental Plant Physiology
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