CHARACTERISTICS FOR SOME RABBIT HEMORRHAGIC DISEASE VIRUS (RHDV) STRAINS PROPAGATED IN ISOLATED RENAL CELL OF RABBIT

Taiwan Veterinary Journal Pub Date : 2024-03-09 DOI:10.1142/s168264852450001x

Hyok Ryo, Yong-Mi Pyon, Hi-Pung Pak, Sun-Nam Pak

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Abstract

The renal cells were isolated from the kidney of different week-age of healthy rabbits by a single two-stage pass perfusion method and suspended in Hanks’ balanced salt solution (Ca[Formula: see text]-, Mg[Formula: see text]-free). The field strains of RHDV were inoculated into the suspension and incubated for 4 days at 37∘C. After incubation, the HA titer of each strain of RHDV in supernatant of virus growth was measured using human erythrocyte O type. There were differences in the HA titers of RHDV strains according to the renal cell of different week-age of rabbits, the number of hepatocyte, the strain of virus (RHDV-1, RHDV-2, RHDV-3) and the duration of incubation. The HA titer was highest when the virus was cultured in the renal cells isolated from more than 12 week-aged rabbits. And the HA titer of RHDV propagated in isolated renal cell was higher than hepatocytes. When immunized rabbits by inactivated virus strains (RHDV-1, RHDV-2 and RHDV-3), two strains, RHDV-1 and RHDV-3, showed the same level of HI titers with inactivated homogenate, but RHDV-2 did lower level of titer than the homogenate.

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在兔离体肾细胞中繁殖的一些兔出血性疾病病毒（rhdv）毒株的特征

采用单次两段式灌流法从不同周龄的健康家兔肾脏中分离出肾细胞，并将其悬浮于 Hanks 平衡盐溶液（不含 Ca[式：见正文]，Mg[式：见正文]）中。将 RHDV 实地菌株接种到悬浮液中，在 37∘C 孵育 4 天。培养结束后，用人红细胞 O 型测定病毒生长上清液中各 RHDV 株系的 HA 滴度。不同周龄兔子的肾细胞、肝细胞数量、病毒株（RHDV-1、RHDV-2、RHDV-3）和培养时间对 RHDV 株系的 HA 滴度有差异。从 12 周龄以上的兔子分离的肾细胞培养病毒时，HA 滴度最高。在分离的肾细胞中繁殖的 RHDV 的 HA 滴度高于肝细胞。用灭活病毒株（RHDV-1、RHDV-2 和 RHDV-3）免疫兔子时，RHDV-1 和 RHDV-3 两株的 HI 滴度与灭活匀浆的滴度相同，但 RHDV-2 的滴度低于匀浆。

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Taiwan Veterinary Journal

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