{"title":"Drosophila Tak1 Mediates the Condensation and Deubiquitinase Activity of Otu in a Kinase-Independent Manner","authors":"Chuchu Zhang, Xueli Shao, Zhuolan Xia, Yihua Xiao, Baoyi Hu, Yulong Yang, Xiangyun Li, Zhen Zhang, Abdul Qadeer, Yangyang Zhu","doi":"10.1134/s1062359023605013","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>In the intricate molecular world, post-translational modifications play pivotal roles in modulating protein activity and function. The <i>Drosophila</i> <i>melanogaster</i> genome encodes an array of enzymes to mediate these processes. Here, we elucidate a previously unrecognized connection between the <i>Drosophila</i> transforming growth factor-β-activated kinase 1 (dTak1) and the deubiquitinase Ovarian tumor (Otu). We demonstrate that dTak1 mediates both the phase-separated condensation and the deubiquitinase enzymatical activity of Otu in a manner independent of its kinase function. Using a combination of biochemical assays and in vivo genetic interactions, our findings highlight the significance of dTak1 in orchestrating the assembly of Otu condensates, and consequently, in the regulation of Otu’s biological functions. Our studies underscore the multifaceted nature of protein-protein interactions and open up avenues for exploring non-traditional roles of kinases in cellular processes.</p>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":null,"pages":null},"PeriodicalIF":0.5000,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biology Bulletin","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s1062359023605013","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
In the intricate molecular world, post-translational modifications play pivotal roles in modulating protein activity and function. The Drosophilamelanogaster genome encodes an array of enzymes to mediate these processes. Here, we elucidate a previously unrecognized connection between the Drosophila transforming growth factor-β-activated kinase 1 (dTak1) and the deubiquitinase Ovarian tumor (Otu). We demonstrate that dTak1 mediates both the phase-separated condensation and the deubiquitinase enzymatical activity of Otu in a manner independent of its kinase function. Using a combination of biochemical assays and in vivo genetic interactions, our findings highlight the significance of dTak1 in orchestrating the assembly of Otu condensates, and consequently, in the regulation of Otu’s biological functions. Our studies underscore the multifaceted nature of protein-protein interactions and open up avenues for exploring non-traditional roles of kinases in cellular processes.
期刊介绍：
Biology Bulletin (Izvestiya Rossiiskoi Akademii Nauk – Seriya Biologicheskaya) is an interdisciplinary journal of general biology. It focuses on fundamental studies in the fields of cell biology, biochemistry, zoology, botany, physiology, and ecology. This journal publishes current materials of experimental studies and surveys on current problems in general biology. It also publishes information on scientific conferences and new books in the fields of general biology.
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