Protective Effect of Ginkgo biloba (Ginkgoaceae Family) Leaf Extracts on Female Drosophila melanogaster Suffering from Ultraviolet Irradiation through Activating the Keap1-Nrf2 Signaling Pathway

IF 0.5 4区生物学 Q4 BIOLOGY Biology Bulletin Pub Date : 2024-03-26 DOI:10.1134/s1062359023604159

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Abstract

Ginkgo biloba leaf extracts (GBLE) possess strong physiological activities, such as antioxidant and anti-inflammatory effects. In the experiment, the mitigating effects of GBLE on ultraviolet (UV) radiation-induced damage were studied in female Drosophila melanogaster (D. melanogaster). Based on the concentration screening experiments, GBLE was finally added to the food medium at a concentration of 0.04 mg/mL to ameliorate the damage to female D. melanogaster suffering from the irradiating dose (300 μW/cm³, 40 cm, 50 min). The results indicated that the mean lifespan of D. melanogaster fed GBLE food was increased by 5.80% in comparison with that of the control group, and the climbing ability was increased by 69.79% with antioxidant capacity improvement, including a reduction in ROS levels in the fat body. Additionally, GBLE could also promote the recovery of cyclogeny and fertility of UV-damaged D. melanogaster. The gene expression levels in the Keap1-Nrf2 signaling pathway were significantly promoted in female D. melanogaster suffering from UV radiation with GBLE supplementation, and according to the Nrf2-RNAi D. melanogaster experiments, GBLE ameliorated the effect of UV damage through activating the Keap1-Nrf2 signaling pathway.

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银杏叶提取物通过激活 Keap1-Nrf2 信号通路对遭受紫外线照射的雌果蝇的保护作用

摘要银杏叶提取物（GBLE）具有很强的生理活性，如抗氧化和抗炎作用。本实验研究了银杏叶提取物对紫外线辐射（UV）引起的雌性黑腹果蝇（D. melanogaster）损伤的缓解作用。根据浓度筛选实验，最终在食物培养基中添加浓度为0.04 mg/mL的GBLE，以改善雌性黑腹果蝇在辐照剂量（300 μW/cm3, 40 cm, 50 min）下的损伤。结果表明，与对照组相比，喂食GBLE食物的雌性黑腹滨蝽的平均寿命延长了5.80%，爬行能力提高了69.79%，抗氧化能力也有所提高，包括脂肪体中ROS水平的降低。此外，GBLE 还能促进受紫外线损伤的黑腹蝇的周期发生和繁殖能力的恢复。补充GBLE后，遭受紫外线辐射的雌性黑腹滨蝽体内Keap1-Nrf2信号通路的基因表达水平明显提高，根据Nrf2-RNAi黑腹滨蝽实验，GBLE通过激活Keap1-Nrf2信号通路改善了紫外线损伤的影响。

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来源期刊

Biology Bulletin 生物-生物学

CiteScore

0.70

自引率

20.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Biology Bulletin (Izvestiya Rossiiskoi Akademii Nauk – Seriya Biologicheskaya) is an interdisciplinary journal of general biology. It focuses on fundamental studies in the fields of cell biology, biochemistry, zoology, botany, physiology, and ecology. This journal publishes current materials of experimental studies and surveys on current problems in general biology. It also publishes information on scientific conferences and new books in the fields of general biology.