Improved point-of-care detection of P. gingivalis using optimized surface-enhanced Raman scattering in lateral flow assays†

IF 3.5 Q2 CHEMISTRY, ANALYTICAL Sensors & diagnostics Pub Date : 2024-03-28 DOI:10.1039/D4SD00056K
Lyndsay N. Kissell, Daewoo Han, Der Vang, Alexander W. R. Cikanek, Andrew J. Steckl and Pietro Strobbia
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Abstract

The introduction of surface-enhanced Raman scattering (SERS) in lateral flow assays (LFA) has been recently exploited to increase the sensitivity and quantification capabilities of these rapid tests. Herein, we took advantage of a SERS–LFA combination to improve LFAs designed to test for P. gingivalis, a biomarker for oral health. We have demonstrated a limit of detection (LOD) < 10 ng mL−1, which is within the range of concentration needed to monitor oral health. By comparison, conventional colorimetric LFAs achieve an LOD ≈ 100 ng mL−1, generating a lower diagnostic sensitivity. To achieve this enhanced sensitivity, we optimized the materials used in the SERS–LFA, investigating nanostars (NS) differing in size and material composition and comparing them to commercial gold nanoparticles, as a benchmark. We found that large (56 nm) NSs with a silver coating were the most sensitive nanomaterials for SERS–LFA. To prove the applicability of this SERS–LFA to point-of-care (POC) settings, we tested the optimized LFA with a portable Raman system prototype designed to work on LFAs with 3D-printed cartridges and a line-shaped laser illumination. Using this prototype, we achieved the same LOD observed with the traditional benchtop Raman system. The use of a portable Raman system has brought the SERS–LFA technology closer to the POC use.

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利用优化的表面增强拉曼散射技术在侧流检测中改进牙龈脓疱病的护理点检测
最近,在侧流检测法(LFA)中引入了表面增强拉曼散射(SERS)技术,以提高这些快速检测方法的灵敏度和定量能力。在这里,我们利用 SERS-LFA 组合的优势,改进了用于检测牙龈脓疱病(一种口腔健康生物标志物)的 LFA。我们已证明检测限(LOD)为 10 纳克/毫升,在监测口腔健康所需的浓度范围内。相比之下,传统比色法 LFA 的检测限为 100 纳克/毫升,诊断灵敏度较低。为了提高灵敏度,我们对 SERS-LFA 中使用的材料进行了优化,研究了不同尺寸和材料成分的纳米星(NS),并将它们与商用金纳米颗粒进行了比较。我们发现,具有银涂层的大型(56 纳米)NS 是 SERS-LFA 中灵敏度最高的纳米材料。为了证明这种 SERS-LFA 在护理点(POC)环境中的适用性,我们使用便携式拉曼系统原型对优化的 LFA 进行了测试,该原型设计用于带有 3D 打印盒和线形激光照明的 LFA。使用该原型,我们达到了与传统台式拉曼系统相同的 LOD 值。便携式拉曼系统的使用使 SERS-LFA 技术更接近于 POC 使用。
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Back cover Pursuing theranostics: a multimodal architecture approach. A review on Ti3C2Tx based nanocomposites for the electrochemical sensing of clinically relevant biomarkers Back cover Introduction to Supramolecular Sensors: From Molecules to Materials
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