Caroline Mitiká Watanabe, Nathália Almeida Souza Tancler, Aline Márcia Marques Braz, Shelly Favorito de Carvalho, Giovanni Faria Silva, Maria Inês de Moura Campos Pardini, Maria Aparecida Custódio Domingues, Guilherme Targino Valente, Paulo Eduardo de Abreu Machado, Rejane Maria Tommasini Grotto, Marjorie de Assis Golim
{"title":"Hepatitis C Virus Infected Human Megakaryocytes and Platelets: Intra-and Extracellular Evaluation","authors":"Caroline Mitiká Watanabe, Nathália Almeida Souza Tancler, Aline Márcia Marques Braz, Shelly Favorito de Carvalho, Giovanni Faria Silva, Maria Inês de Moura Campos Pardini, Maria Aparecida Custódio Domingues, Guilherme Targino Valente, Paulo Eduardo de Abreu Machado, Rejane Maria Tommasini Grotto, Marjorie de Assis Golim","doi":"10.34257/gjmrcvol24is1pg1","DOIUrl":null,"url":null,"abstract":"This study aimed to determine whether the hepatitis C virus (HCV) infects human megakaryocytes and platelets and to measure the expression of receptors involved in virus-cell interaction. Platelets from healthy donors were infected with HCV in vitro and analyzed for viral expression and the receptors claudin-1 and cluster of differentiation 81 (CD81). HCV was detected on the surface and cytoplasm of both cells; cytoplasmic expression was higher compared to the surface. Platelets presented a claudin-1+/CD81-phenotype, and megakaryocytes showed aclaudin-1+/CD81+ phenotype. We conclude that megakaryocytes and platelets are susceptible to HCV infection, regardless of CD81 expression, and megakaryocytes may serve as possible sites of viral replication.","PeriodicalId":93101,"journal":{"name":"Global journal of medical research","volume":"34 5","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Global journal of medical research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.34257/gjmrcvol24is1pg1","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
This study aimed to determine whether the hepatitis C virus (HCV) infects human megakaryocytes and platelets and to measure the expression of receptors involved in virus-cell interaction. Platelets from healthy donors were infected with HCV in vitro and analyzed for viral expression and the receptors claudin-1 and cluster of differentiation 81 (CD81). HCV was detected on the surface and cytoplasm of both cells; cytoplasmic expression was higher compared to the surface. Platelets presented a claudin-1+/CD81-phenotype, and megakaryocytes showed aclaudin-1+/CD81+ phenotype. We conclude that megakaryocytes and platelets are susceptible to HCV infection, regardless of CD81 expression, and megakaryocytes may serve as possible sites of viral replication.
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