Assessment of Anti-inflammatory, Antimicrobial and Cytotoxicity of Chitosan-Moringa Composite and Calcium Hydroxide Nanoparticles as an intra-canal medicament in vitro

Abstract

In this study Chitosan nanoparticles was characterized usingUV spectrophotometry, FT-IR, Transmission electron microscopy, and X-Ray diffraction. The composition of Moringa oleifera of ethanolic extract was analyzed using GC-Mass.The antimicrobial, anti-inflammatory, and cytotoxicity of Chitosan-Moringa composite, H2CaO2 nanoparticles, Ca(OH)2, and Moringa oleifera of ethanol extract as an intra-canal medicament in vitro were also investigated. Results of our research summarized that; The UV of chitosan nanoparticles range from 280 to 300 nm. The FT-IR results confirm the presence of a broad and powerful band at 3442 cm1, 1636 cm1, and 1052 cm1 all band confirm the presence of the native chitosan. The X-Ray diffraction proved three strong characteristic peaks indicating crystallinity of chitosan nanoparticles chains. The TEM of Chitosan nanoparticles size was between 76.61 – 126.91nm and the shape was less spherical with slightly wrinkled surface. The major chemical compounds in Moringa ethanol extract by GC-Mass were detected. Also, the antimicrobial activity of Moringa extracts proved that ethanol extract had the highest zone of inhibition. The antimicrobial activity of Moringa-chitosan composite had the highest antimicrobial activity followed by H2CaO2 nanoparticles against dental pathogens. The anti-inflammatory effects on HRBC hemolysis at concentration 100μg/mL Ca (OH)2 gave the best lower production than the positive control Stander Indo meth followed by H2CaO2 nanoparticles, Moringa ethanol extract, and finally Chitosan-Moringa composite. The MTT assay against OEC have been showed that, Ca (OH)2 is the most cytotoxic with the lowest IC50 followed by Moringa ethanol extract, Chitosan-Moringa composite, and finally H2CaO2 nanoparticles.