Simultaneous determination of Cefepime and Tazobactam by using Hyphenated Liquid Chromatography (UPLC-MS/MS)

Abstract

The present study deals with establishing a hyphenated analytical method and validating the same for the determination of the drug combination of Cefepime and Tazobactam simultaneously by UPLC-MS/MS. A C18 BEH analytical column was employed. A mobile phase of 0.2 percent formic acid in water and acetonitrile were used in a 20:80 v/v ratio. Samples are ionized using +ESI ionization and detected in MRM mode. The retention time of 0.80 min and 0.96 min were observed for Cefepime and Tazobactam respectively. The drugs have linear responses in the investigated range, with r2 = 0.9955 for Cefepime and r2 = 0.9816 for Tazobactam with increased precision and accuracy. The LOD for Cefepime and Tazobactam was 66ng/ml and 8.3ng/ml respectively. The LOQ for Cefepime and Tazobactam was 200ng/ml and 25ng/ml respectively. According to the findings, the hyphenated UPLC-MS/MS method was developed and validated as per ICH regulations to determine Cefepime and Tazobactam simultaneously.