In vitro Propagation of Ocimum Sanctum Linn by Using Growth Hormone Shoot Induction

Abstract

The majority of Indian homes include tulsi, often known as holy basil (Ocimum sanctum L). Although it is regarded as a spiritual plant, physiologically speaking, it is one of the most readily available antibiotics. Tulsi is the most significant herb in Ayurveda, and current research is confirming its health advantages. It is also one of the plants that are utilised widely in Ayurvedic treatments.It relieves physical, physiological, metabolic, and psychological stress due to its special mix of pharmacological activity. A substrate for the quick development and multiplication of commercially significant plants is provided by plant tissue culture. Determining the optimal explants type and medium conditions for large-scale in vitro Tulsi shoot induction is the aim of the current effort. In the current investigation, the nodal segment and shoot tips were employed as explants. The nodal segment reacted well with a frequency rate of about 90% on all MS media utilized in the current investigation, including media with BAP and media with different combinations of BAP and IAA. It was seen that one or more shoots were emerging from the explants' nodal area after 10 days of culture. In this work, we examined the effects of various PGR combinations and dosages on the in vitro micro propagation of Tulsi, a fragrant and therapeutic plant (Ocmium sanctum L.). Three distinct PGRs were utilized, namely 6-benzylaminopurine (BAP), naphthalene acetic acid (NAA), and indole-3-acetic acid (IAA). The optimal medium for inducing and multiplying shoots was found to be Murashige and Skoog (MS) medium supplemented with 0.25 mg/l BAP and 0.1 mg/l NAA. The MS medium exhibits average shoot formation with 0.025 mg/l IAA and 0.1 mg/l BAP. Our results demonstrate that Tulsi may be successfully micro-propagated in vitro with the appropriate PGR.