Development of micropropagation protocol, assessment of genetic fidelity and phytochemical analysis of Blepharispermum subsessile DC.: a conservation concerned medicinal plant of Eastern Ghats, India

IF 2.3 3区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Plant Cell, Tissue and Organ Culture Pub Date : 2024-04-06 DOI:10.1007/s11240-024-02747-z

Kumari Monalisa, Shashikanta Behera, Siba P. Pidika, Sanjay K. Madkami, Soumendra K. Naik

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Abstract

Blepharispermum subsessile DC. is well known for its ethnomedicinal values. This plant has been used traditionally for the treatment of various diseases. Due to over-exploitation, habitat destruction, and climate change B. subsessile has been included in the list as threatened plants. Hence urgent attention is needed for the protection and conservation of this endangered plant. This plant is found in ‘Gurudangar’, Odisha, India and it is declared as Medicinal Plant Conservation Areas for in situ conservation of this plant species along with few other medicinal plants. In vitro propagation is an extremely effective alternative to overcome the limitations of conventional propagation methods and to escalate the production of B. subsessile. Keeping this in view, the present study was envisaged to develop an efficient protocol for in vitro plant regeneration of B. subsessile from cotyledonary node explant. Multiple shoots were induced from the cotyledonary node on Murashige and Skoog’s (1962) (MS) medium supplemented with different types and concentrations of plant growth regulators. A combined effect of 2.0 mg/L meta-Topolin (mT) and 1.0 mg/L indole-3-acetic acid (IAA) when augmented with MS medium was evaluated as optimum for multiple in vitro shoot regeneration from cotyledonary node. Further, in vitro nodal segments were inoculated on different plant growth regulator supplemented medium for upscaling and mT (2.0 mg/L) was found to be best for such in vitro shoot proliferation. The in vitro shoots were rooted on ½ MS medium supplemented with 0.5 mg/L indole-3-butyric acid (IBA). The in vitro regenerated plants were successfully acclimatized in the small pots containing sterile garden soil and sand (1:1) followed by the transfer to larger pots containing garden soil. The genetic fidelity of in vitro regenerated plants was assessed and ascertained by ISSR markers. The phytochemical analysis and antioxidant activity of the in vitro regenerated plants vis-à-vis mother plant were also evaluated to find out the biochemical fidelity.

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印度东高止山脉药用植物 Blepharispermum subsessile DC.的微繁殖方案开发、遗传保真度评估和植物化学分析

摘要 Blepharispermum subsessile DC.以其民族药用价值而闻名。这种植物在传统上被用于治疗各种疾病。由于过度开发、栖息地遭到破坏以及气候变化，B. subsessile 已被列入濒危植物名单。因此，保护和养护这种濒危植物刻不容缓。这种植物分布在印度奥迪沙邦的 "古鲁丹加"，该地区已被宣布为药用植物保护区，用于就地保护这种植物物种和其他一些药用植物。体外繁殖是一种极其有效的替代方法，可以克服传统繁殖方法的局限性，提高亚细叶蝙蝠蛾的产量。有鉴于此，本研究旨在开发一种有效的子叶节外植体离体再生 B. subsessile 的方法。在添加了不同类型和浓度植物生长调节剂的 Murashige 和 Skoog（1962 年）（MS）培养基上，从子叶节诱导出多个芽。在 MS 培养基中添加 2.0 毫克/升甲基托布津（mT）和 1.0 毫克/升吲哚-3-乙酸（IAA）的综合效果被认为是子叶节离体再生多芽的最佳选择。此外，还将离体节段接种到添加了不同植物生长调节剂的培养基上进行增殖，结果发现 mT（2.0 毫克/升）对这种离体芽增殖效果最佳。离体芽在添加了 0.5 毫克/升吲哚-3-丁酸（IBA）的 ½ MS 培养基上生根。离体再生植株在装有无菌园土和沙子（1:1）的小盆中成功地适应了环境，然后转移到装有园土的大盆中。通过 ISSR 标记评估并确定了离体再生植株的遗传保真度。还对离体再生植株与母株的植物化学分析和抗氧化活性进行了评估，以确定其生化保真度。

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来源期刊

Plant Cell, Tissue and Organ Culture 生物-生物工程与应用微生物

CiteScore

5.40

自引率

13.30%

发文量

203

审稿时长

3.3 months

期刊介绍： This journal highlights the myriad breakthrough technologies and discoveries in plant biology and biotechnology. Plant Cell, Tissue and Organ Culture (PCTOC: Journal of Plant Biotechnology) details high-throughput analysis of gene function and expression, gene silencing and overexpression analyses, RNAi, siRNA, and miRNA studies, and much more. It examines the transcriptional and/or translational events involved in gene regulation as well as those molecular controls involved in morphogenesis of plant cells and tissues. The journal also covers practical and applied plant biotechnology, including regeneration, organogenesis and somatic embryogenesis, gene transfer, gene flow, secondary metabolites, metabolic engineering, and impact of transgene(s) dissemination into managed and unmanaged plant systems.