E. Héctor, D. Cevallos, L. Corozo, F. Macías, O. Fosado
{"title":"Development of a protocol for the micropropagation of two forest species threatened with extinction in Ecuador","authors":"E. Héctor, D. Cevallos, L. Corozo, F. Macías, O. Fosado","doi":"10.1007/s11240-024-02864-9","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p><i>Handroanthus chrysanthus</i> Jacq. S. O. Grose and <i>Tabebuia rosea</i> (Bertol.) Bertero ex A.DC are two forest species that grow in the coastal region of Ecuador and are threatened with extinction. A protocol for the mass multiplication of these species was developed using in vitro culture techniques. The cultures were initiated from seeds, and the effect of two culture media: Woody Plant Medium (WPM) and Murashige-Skoog (MS), two concentrations of NaClO (0.5% and 1%), and two disinfection times (3 and 5 min) was studied. During multiplication, the effect of three concentrations of two cytokinins: 6-benzylaminopurine (6-BAP) 4.4, 5.5, or 6.6 μM; kinetin 4.6, 5.75, or 6.9 μM) on the number of shoots, their length, and diameter was analyzed. This phase of the experiment was carried out in two successive multiplications. For rooting, two concentrations of indole-3-butyric acid (IBA) (2.45 and 4.9 μM) were tested, and the number of roots formed and their length were determined. It was demonstrated that the WPM medium is the most suitable for the in vitro culture of both species and that disinfection time and NaClO concentration affect each species differently. For the multiplication of <i>H. chrysanthus</i>, the most suitable cytokinin was 6-BAP 6.6 μM; <i>T. rosea</i> performed better in the absence of cytokinins. IBA 2.45 μM produced the best results for the rooting of <i>H. chrysanthus</i>, while for <i>T. rosea</i>, IBA 4.9 μM was the most suitable. The acclimatized plants showed a high survival rate, demonstrating the feasibility of using this methodology for the accelerated propagation of these endangered species.</p><h3 data-test=\"abstract-sub-heading\">Key message</h3><p>In this research, the culture medium requirements and conditions for the micropropagation of <i>H. chrysanthus</i> and <i>T. rosea</i> were fine-tuned. This technique can be implemented to obtain plants for use in reforestation.</p>","PeriodicalId":20219,"journal":{"name":"Plant Cell, Tissue and Organ Culture","volume":"39 1","pages":""},"PeriodicalIF":2.3000,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Cell, Tissue and Organ Culture","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s11240-024-02864-9","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Handroanthus chrysanthus Jacq. S. O. Grose and Tabebuia rosea (Bertol.) Bertero ex A.DC are two forest species that grow in the coastal region of Ecuador and are threatened with extinction. A protocol for the mass multiplication of these species was developed using in vitro culture techniques. The cultures were initiated from seeds, and the effect of two culture media: Woody Plant Medium (WPM) and Murashige-Skoog (MS), two concentrations of NaClO (0.5% and 1%), and two disinfection times (3 and 5 min) was studied. During multiplication, the effect of three concentrations of two cytokinins: 6-benzylaminopurine (6-BAP) 4.4, 5.5, or 6.6 μM; kinetin 4.6, 5.75, or 6.9 μM) on the number of shoots, their length, and diameter was analyzed. This phase of the experiment was carried out in two successive multiplications. For rooting, two concentrations of indole-3-butyric acid (IBA) (2.45 and 4.9 μM) were tested, and the number of roots formed and their length were determined. It was demonstrated that the WPM medium is the most suitable for the in vitro culture of both species and that disinfection time and NaClO concentration affect each species differently. For the multiplication of H. chrysanthus, the most suitable cytokinin was 6-BAP 6.6 μM; T. rosea performed better in the absence of cytokinins. IBA 2.45 μM produced the best results for the rooting of H. chrysanthus, while for T. rosea, IBA 4.9 μM was the most suitable. The acclimatized plants showed a high survival rate, demonstrating the feasibility of using this methodology for the accelerated propagation of these endangered species.
Key message
In this research, the culture medium requirements and conditions for the micropropagation of H. chrysanthus and T. rosea were fine-tuned. This technique can be implemented to obtain plants for use in reforestation.
期刊介绍：
This journal highlights the myriad breakthrough technologies and discoveries in plant biology and biotechnology. Plant Cell, Tissue and Organ Culture (PCTOC: Journal of Plant Biotechnology) details high-throughput analysis of gene function and expression, gene silencing and overexpression analyses, RNAi, siRNA, and miRNA studies, and much more. It examines the transcriptional and/or translational events involved in gene regulation as well as those molecular controls involved in morphogenesis of plant cells and tissues.
The journal also covers practical and applied plant biotechnology, including regeneration, organogenesis and somatic embryogenesis, gene transfer, gene flow, secondary metabolites, metabolic engineering, and impact of transgene(s) dissemination into managed and unmanaged plant systems.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
