Liquid in vitro culture system allows gradual intensification of osmotic stress in Solanum tuberosum through sorbitol

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-04-02 DOI:10.1007/s11240-024-02720-w

Katharina Wellpott, Marco Herde, Traud Winkelmann, Christin Bündig

{"title":"Liquid in vitro culture system allows gradual intensification of osmotic stress in Solanum tuberosum through sorbitol","authors":"Katharina Wellpott, Marco Herde, Traud Winkelmann, Christin Bündig","doi":"10.1007/s11240-024-02720-w","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Because of their shallow root system, drought stress is a major problem in potato cultivation. Due to climate change more severe drought periods are expected to occur in the vegetative growth phase of potato growth. Therefore, there is a great need for drought tolerant potato genotypes. Potato responds to drought stress in the field in various ways, including osmoregulation. Osmotic stress can be induced in vitro by adding an osmotic agent and thus lowering the osmotic potential of the medium. In this study, a new, cost-effective in vitro test system is presented, in which the osmotic agent can be gradually added after root formation to prevent an osmotic shock. This is achieved by using sieves as plant holders and liquid medium, which, allows an improved simulation of gradually drying soil. Responses to osmotic stress in four potato genotypes were analysed and an increase in proline under osmotic stress was detected. Moreover, genes of interest that were postulated to be linked to drought stress were shown by quantitative qRT-PCR to be regulated under osmotic stress. Furthermore, we showed that the content of sorbitol, which was used as osmotic agent, was 700- fold higher for ‘Eurostarch’ after seven days under osmotic stress and 1093- fold higher after 14 days, respectively, compared to control plants without sorbitol addition. Therefore, further investigations must show, whether it was taken up through the roots, is metabolised, stored or de novo synthesised by the potato plants.</p><h3 data-test=\"abstract-sub-heading\">Keypoints</h3><p>The established novel in vitro test system for potato allows gradually increasing stress exposition of rooted plants. Sorbitol seems not an ideal osmotic agent as it is likely taken up.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s11240-024-02720-w","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}

引用次数: 0

Abstract

Because of their shallow root system, drought stress is a major problem in potato cultivation. Due to climate change more severe drought periods are expected to occur in the vegetative growth phase of potato growth. Therefore, there is a great need for drought tolerant potato genotypes. Potato responds to drought stress in the field in various ways, including osmoregulation. Osmotic stress can be induced in vitro by adding an osmotic agent and thus lowering the osmotic potential of the medium. In this study, a new, cost-effective in vitro test system is presented, in which the osmotic agent can be gradually added after root formation to prevent an osmotic shock. This is achieved by using sieves as plant holders and liquid medium, which, allows an improved simulation of gradually drying soil. Responses to osmotic stress in four potato genotypes were analysed and an increase in proline under osmotic stress was detected. Moreover, genes of interest that were postulated to be linked to drought stress were shown by quantitative qRT-PCR to be regulated under osmotic stress. Furthermore, we showed that the content of sorbitol, which was used as osmotic agent, was 700- fold higher for ‘Eurostarch’ after seven days under osmotic stress and 1093- fold higher after 14 days, respectively, compared to control plants without sorbitol addition. Therefore, further investigations must show, whether it was taken up through the roots, is metabolised, stored or de novo synthesised by the potato plants.

Keypoints

The established novel in vitro test system for potato allows gradually increasing stress exposition of rooted plants. Sorbitol seems not an ideal osmotic agent as it is likely taken up.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

液体离体培养系统可通过山梨醇逐步增强块茎茄的渗透胁迫

摘要由于马铃薯的根系较浅，干旱胁迫是马铃薯种植中的一个主要问题。由于气候变化，在马铃薯的无性生长阶段预计会出现更严重的干旱期。因此，亟需耐旱的马铃薯基因型。马铃薯在田间以各种方式对干旱胁迫做出反应，包括渗透调节。渗透胁迫可通过添加渗透剂从而降低培养基的渗透势在体外诱导。本研究提出了一种新的、具有成本效益的体外测试系统，其中渗透剂可在根系形成后逐渐添加，以防止渗透休克。这是通过使用筛子作为植物支架和液体介质来实现的，这样可以更好地模拟逐渐干燥的土壤。分析了四种马铃薯基因型对渗透胁迫的反应，发现脯氨酸在渗透胁迫下有所增加。此外，通过定量 qRT-PCR 技术还发现，与干旱胁迫有关的相关基因在渗透胁迫下受到调控。此外，我们还发现，与未添加山梨醇的对照植株相比，'Eurostarch'在渗透胁迫下7天后山梨醇含量增加了700倍，14天后增加了1093倍。因此，进一步的研究必须表明山梨醇是通过根部吸收、代谢、储存还是由马铃薯植株重新合成的。山梨醇似乎不是理想的渗透剂，因为它很可能被吸收。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊