Influence of Silver Nanoparticle Supplementation on Growth Performance, Immune Response, Tissue Biopsy, and Gene Transcription in the Aeromonas carviae challenged Labeo rohita

Abstract

The study examined the impact of a diet enriched with nanosilver (AgNPs) on the specific immunity, tissue biopsy, and growth effectiveness of Labeo rohita infected with A. carviae. The fish were divided into four groups with replications, and three diets were prepared with AgNPs (0, 10, 15, and 20 μg AgNPs kg-1 diet) added. The fish in the 15μg kg-1 group showed improved growth rates and a 70% survival rate after being challenged with A. carviae. Antioxidant indices, as well as the non-specific immune response, provided notable variations in the inclusion values of AgNPs in the liver and gill. The supplementation of 15μgkg-1 AgNPs most effectively improved tissue damage caused by A. caviae exposure in immunoprotective organs. T3 had higher liver TNF-α transcription than other treatments, while T4 had the highest values for IL-10. The amount of IL-10 in kidneys, gills, and muscles was also higher in T3, T2 and T3 respectively. The research suggests that AgNPs can positively influence fish growth and manage A. caviae infections by boosting antioxidant status and immunity while causing minimal harm to the immunoprotective organs.