Antihypertensive, Antidiabetic, and Antioxidant Properties of Novel Azolla pinnata Fern Protein Hydrolysates: Inhibition Mechanism, Stability, Profiling, and Molecular Docking

IF 5.3 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Food and Bioprocess Technology Pub Date : 2024-04-27 DOI:10.1007/s11947-024-03412-1

Mohammed S. Qoms, Brisha Arulrajah, Wan Zunairah Wan Ibadullah, Nurul Shazini Ramli, Rosnah Shamsudin, De-Ming Chau, Nazamid Saari

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Abstract

The Azolla pinnata fern protein was enzymatically hydrolysed with Alcalase, Flavourzyme, and Papain at varying degrees of hydrolysis (10, 20, and 30%) to generate multi-biologically active protein hydrolysates. The extensively hydrolysed (30%) Alcalase-generated hydrolysate (AFPH-AE) was most active demonstrating antihypertensive (ACE inhibition), antidiabetic (DPP-IV, α-glucosidase, and α-amylase inhibition), and antioxidant (DPPH, ABTS, and FRAP) activities. AFPH-AE exhibited an uncompetitive inhibition mode against ACE and α-glucosidase, a mixed inhibition mode against α-amylase, and a noncompetitive inhibition mode against DPP-IV. This hydrolysate was highly stable under different food processing conditions including pH 5, 7, and 8, NaCl up to 150 mM, and a high temperature of 100 °C. The low molecular weight fraction (< 3 kDa) exhibited high biological activities, and a total of 15 low molecular weight bioactive peptides were identified. Molecular docking revealed that the peptide-enzyme interactions were mainly mediated via hydrogen bonds and hydrophobic interactions. Overall, these findings reveal the potential of AFPH-AE as a functional and/or nutraceutical ingredient with multifunctional antihypertensive, antidiabetic, and antioxidant effects.

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新型羽扇豆蕨类蛋白质水解物的抗高血压、抗糖尿病和抗氧化特性：抑制机制、稳定性、剖析和分子对接

用钙化酶、黄酮酶和木瓜蛋白酶对羽扇豆蕨类蛋白质进行不同程度的水解（10%、20% 和 30%），生成具有多种生物活性的蛋白质水解物。经大量水解（30%）的阿尔卡拉酶生成的水解物（AFPH-AE）活性最高，具有抗高血压（抑制 ACE）、抗糖尿病（抑制 DPP-IV、α-葡萄糖苷酶和 α-淀粉酶）和抗氧化（DPPH、ABTS 和 FRAP）活性。AFPH-AE 对 ACE 和 α 葡萄糖苷酶具有非竞争性抑制作用，对 α 淀粉酶具有混合抑制作用，对 DPP-IV 具有非竞争性抑制作用。这种水解物在不同的食品加工条件下（包括 pH 值为 5、7 和 8，氯化钠高达 150 mM，以及 100 °C 的高温）都非常稳定。低分子量部分（< 3 kDa）具有很高的生物活性，共鉴定出 15 种低分子量生物活性肽。分子对接显示，肽与酶的相互作用主要是通过氢键和疏水作用介导的。总之，这些发现揭示了 AFPH-AE 作为一种具有多功能降压、抗糖尿病和抗氧化作用的功能性和/或营养保健成分的潜力。

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来源期刊

Food and Bioprocess Technology 农林科学-食品科技

CiteScore

9.50

自引率

19.60%

发文量

200

审稿时长

2.8 months

期刊介绍： Food and Bioprocess Technology provides an effective and timely platform for cutting-edge high quality original papers in the engineering and science of all types of food processing technologies, from the original food supply source to the consumer’s dinner table. It aims to be a leading international journal for the multidisciplinary agri-food research community. The journal focuses especially on experimental or theoretical research findings that have the potential for helping the agri-food industry to improve process efficiency, enhance product quality and, extend shelf-life of fresh and processed agri-food products. The editors present critical reviews on new perspectives to established processes, innovative and emerging technologies, and trends and future research in food and bioproducts processing. The journal also publishes short communications for rapidly disseminating preliminary results, letters to the Editor on recent developments and controversy, and book reviews.