An Association Between GAS5 rs145204276, NEAT1 rs512715, and MEG3 rs4081134 Gene Polymorphisms and Papillary Thyroid Carcinoma.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Reports of Biochemistry and Molecular Biology Pub Date : 2023-10-01 DOI:10.61186/rbmb.12.3.487

Marjan Abdi Pastaki, Saeedeh Salimi, Zahra Heidari, Mohsen Saravani

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Abstract

Background: This study explores the association between growth arrest-specific 5 (GAS5) rs145204276, nuclear paraspeckle assembly transcript 1 (NEAT1) rs512715, and Maternally Expressed 3 (MEG3) rs4081134 polymorphisms and their impact on susceptibility to papillary thyroid carcinoma (PTC), considering differential expression of long noncoding RNAs (lncRNAs) in PTC.

Methods: A case-control study involving 125 papillary thyroid carcinoma (PTC) patients and 125 controls was conducted. Genotyping of polymorphisms was performed using tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) and PCR-restriction fragment length polymorphism (PCR-RFLP) methods.

Results: No significant association was found between the two groups regarding genotypes and allelic frequencies of GAS-5 145204276 and MEG3 rs4081134 polymorphisms. Genetic models also showed the same results. Regarding NEAT1 rs512715, The PTC group had more GC genotypes and over-dominant models of NEAT1 rs512715 than controls, while controls showed a higher frequency of recessive models.

Conclusion: GAS5 rs145204276 and MEG3 rs4081134 polymorphisms showed no significant association with papillary thyroid carcinoma (PTC) risk. In contrast, NEAT1 rs512715 exhibited a significant impact on PTC development.

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GAS5 rs145204276、NEAT1 rs512715 和 MEG3 rs4081134 基因多态性与甲状腺乳头状癌的关系

背景：本研究探讨了生长停滞特异性5（GAS5）rs145204276、核旁组装转录本1（NEAT1）rs512715和母系表达3（MEG3）rs4081134多态性之间的关联及其对甲状腺乳头状癌（PTC）易感性的影响，同时考虑了长非编码RNA（lncRNA）在PTC中的差异表达：方法：对125名甲状腺乳头状癌（PTC）患者和125名对照者进行了病例对照研究。采用四引物扩增难治性突变系统聚合酶链反应（ARMS-PCR）和PCR-限制性片段长度多态性（PCR-RFLP）方法对多态性进行基因分型：结果：两组患者在GAS-5 145204276和MEG3 rs4081134多态性的基因型和等位基因频率方面没有发现明显的关联。遗传模型也显示了相同的结果。关于 NEAT1 rs512715，与对照组相比，PTC 组有更多的 NEAT1 rs512715 GC 基因型和超显性模型，而对照组则显示出更高频率的隐性模型：结论：GAS5 rs145204276和MEG3 rs4081134多态性与甲状腺乳头状癌（PTC）风险无显著关联。结论：GAS5 rs145204276 和 MEG3 rs4081134 多态性与甲状腺乳头状癌（PTC）风险无明显关联，而 NEAT1 rs512715 则对 PTC 的发生有显著影响。

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来源期刊

Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

2.80

自引率

23.50%

发文量

审稿时长

10 weeks

期刊介绍： The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.