Oral administration of Ganoderma lucidum extract protects gingival tissues against proinflammatory TNF and oxidative stress in periodontitis model rats

IF 1.4 4区 生物学 Q4 MYCOLOGY International journal of medicinal mushrooms Pub Date : 2024-05-01 DOI:10.1615/intjmedmushrooms.2024053946
Shahdat Hossain, Mohammad Azizur Rahman, Borhan Uddin, Taslima Nahar, Marzan Sarker, Shoriful Islam Joy
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Abstract

Ganoderma lucidum is a medicinal mushroom that has been tempting since ancient times. We investigated whether chronic oral administration of G. lucidum extract withstands the increases in levels of proinflammatory TNF and lipid peroxide (LPO), an indicator of oxidative stress, in gingival tissue of periodontitis model rats. It was also investigated if G. lucidum extract inhibits oxidative stress induced by Fenton’s reagents in whole homogenates of fresh rat gigival tissues in vitro. Prior to in vivo and in vitro experiments with rats, G. lucidum extract was quantitatively tested for its total polyphenols and/or flavonoids content and ability to scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH)-free radicals. Chronic oral administration of G. lucidum extract (300 mg/kg BW, body weight) significantly reduced TNF and LPO levels in the gingival tissue of periodontitis model rats. G. lucidum extract also inhibited oxidative stress in vitro (P<0.05), as indicated by reduced LPO levels in gingival tissue whole homogenates preincubated with G. lucidum extract. The in vitro results were thus consistent with those in vivo inhibition of lipid peroxidation, DPPH free radical-scavenging effect and the presence of total polyphenols/flavonoids in G. lucidum extract. Our results provide, at least partial evidence for the beneficial effect of G. lucidum on periodontitis, an inflammatory disease of the gums associated with oxidative stress and preceding infectious gum disease.
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口服灵芝提取物可保护牙周炎模型大鼠的牙龈组织免受促炎性 TNF 和氧化应激的影响
灵芝是一种自古以来就很诱人的药用蘑菇。我们研究了长期口服灵芝提取物是否能抑制牙周炎模型大鼠牙龈组织中促炎性 TNF 和氧化应激指标过氧化脂质(LPO)水平的升高。研究还探讨了绿藻提取物是否能抑制Fenton试剂在体外新鲜大鼠牙龈组织全匀浆中诱导的氧化应激。在对大鼠进行体内和体外实验之前,我们定量检测了刺五加提取物的总多酚和/或类黄酮含量以及清除 2,2-二苯基-1-苦基肼(DPPH)自由基的能力。长期口服葫芦巴提取物(300 毫克/千克体重)可显著降低牙周炎模型大鼠牙龈组织中的 TNF 和 LPO 水平。绿巨人提取物还能抑制体外氧化应激(P<0.05),用绿巨人提取物预培养的牙龈组织全匀浆中的 LPO 水平降低就说明了这一点。因此,体外结果与体内的脂质过氧化抑制作用、DPPH 自由基清除作用以及绿巨人提取物中总多酚/类黄酮的存在是一致的。牙周炎是一种牙龈炎症性疾病,与氧化应激和牙龈感染性疾病相关。
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来源期刊
CiteScore
2.60
自引率
16.70%
发文量
91
审稿时长
6-12 weeks
期刊介绍: The rapid growth of interest in medicinal mushrooms research is matched by the large number of disparate groups that currently publish in a wide range of publications. The International Journal of Medicinal Mushrooms is the one source of information that will draw together all aspects of this exciting and expanding field - a source that will keep you up to date with the latest issues and practice. The International Journal of Medicinal Mushrooms published original research articles and critical reviews on a broad range of subjects pertaining to medicinal mushrooms, including systematics, nomenclature, taxonomy, morphology, medicinal value, biotechnology, and much more.
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