{"title":"Elite chemotype selection, antipathogenic activities, secondary metabolite fingerprinting of in vitro regenerated Tinospora cordifolia (Willd.) Hook.f. & Thomson – a plant with multipurpose therapeutic significance","authors":"Avijit Chakraborty, Suproteem Mukherjee, Diptesh Biswas, Indranil Santra, Tarun Halder, Md Mabud Alam, Kumaresh Bera, Biswajit Ghosh","doi":"10.1007/s11240-024-02726-4","DOIUrl":null,"url":null,"abstract":"<p><i>Tinospora cordifolia</i> (Willd.) Hook.f. & Thomson is an ethnomedicinal plant belonging to the family Menispermaceae, known for its multi-disciplinary use. The present study is focused on selecting an elite plant chemotype through phytocompound quantification and antimicrobial activity assay against multi-drug-resistant (MDR) urinary-tract-infecting (UTI) pathogens, being a growing concern for mortality and antimicrobial resistance nowadays. Twelve accessions of this plant were collected from different agro-climatic zones of West Bengal, and crude extraction was done by solvent optimization. Sample TC 07 resulted in maximum crude extract yield in methanol. All twelve sample extracts showed antimicrobial efficacy against eight pathogens producing significant inhibition zones, while TC 07 resulting maximum inhibition zone of 16.37 ± 0.12 mm against <i>Staphylococcus aureus</i>. Extracts were evaluated through high-performance thin-layer chromatography, which resulted in optimum amount of berberine (5.05 ± 0.05 mg/g) and palmatine (3.00 ± 0.11 mg/g) in TC 07, significantly higher compared to other accessions. Therefore, comparing the data, TC 07 was considered as the elite chemotype and introduced in in vitro culture for conservation. The maximum number of shoot (16.39 ± 0.09) production was obtained in MS medium containing 2.0 mg/l 6-Benzylaminopurine and 1.0 mg/l <i>meta</i>-Topolin. The plant was acclimatized in <i>ex-vitro</i> condition and confirmed for optimum bioactive compound production through high-performance liquid chromatography. The cytogenetic stability of the regenerated plantlets was ensured using chromosome number determination, and through start codon targeted (SCoT) polymorphism. Therefore, this study provides a simple, validated and reproducible method for optimal berberine and palmatine production and controlling MDR-UTI pathogens.</p>","PeriodicalId":20219,"journal":{"name":"Plant Cell, Tissue and Organ Culture","volume":"1 1","pages":""},"PeriodicalIF":2.3000,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Cell, Tissue and Organ Culture","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s11240-024-02726-4","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Tinospora cordifolia (Willd.) Hook.f. & Thomson is an ethnomedicinal plant belonging to the family Menispermaceae, known for its multi-disciplinary use. The present study is focused on selecting an elite plant chemotype through phytocompound quantification and antimicrobial activity assay against multi-drug-resistant (MDR) urinary-tract-infecting (UTI) pathogens, being a growing concern for mortality and antimicrobial resistance nowadays. Twelve accessions of this plant were collected from different agro-climatic zones of West Bengal, and crude extraction was done by solvent optimization. Sample TC 07 resulted in maximum crude extract yield in methanol. All twelve sample extracts showed antimicrobial efficacy against eight pathogens producing significant inhibition zones, while TC 07 resulting maximum inhibition zone of 16.37 ± 0.12 mm against Staphylococcus aureus. Extracts were evaluated through high-performance thin-layer chromatography, which resulted in optimum amount of berberine (5.05 ± 0.05 mg/g) and palmatine (3.00 ± 0.11 mg/g) in TC 07, significantly higher compared to other accessions. Therefore, comparing the data, TC 07 was considered as the elite chemotype and introduced in in vitro culture for conservation. The maximum number of shoot (16.39 ± 0.09) production was obtained in MS medium containing 2.0 mg/l 6-Benzylaminopurine and 1.0 mg/l meta-Topolin. The plant was acclimatized in ex-vitro condition and confirmed for optimum bioactive compound production through high-performance liquid chromatography. The cytogenetic stability of the regenerated plantlets was ensured using chromosome number determination, and through start codon targeted (SCoT) polymorphism. Therefore, this study provides a simple, validated and reproducible method for optimal berberine and palmatine production and controlling MDR-UTI pathogens.
期刊介绍：
This journal highlights the myriad breakthrough technologies and discoveries in plant biology and biotechnology. Plant Cell, Tissue and Organ Culture (PCTOC: Journal of Plant Biotechnology) details high-throughput analysis of gene function and expression, gene silencing and overexpression analyses, RNAi, siRNA, and miRNA studies, and much more. It examines the transcriptional and/or translational events involved in gene regulation as well as those molecular controls involved in morphogenesis of plant cells and tissues.
The journal also covers practical and applied plant biotechnology, including regeneration, organogenesis and somatic embryogenesis, gene transfer, gene flow, secondary metabolites, metabolic engineering, and impact of transgene(s) dissemination into managed and unmanaged plant systems.
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