An innovative label-free electrochemical aptamer sensor: utilizing Ti₃C₂T_x/MoS₂/Au NPs for accurate interleukin-6 detection.

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL Talanta Pub Date : 2024-08-15 Epub Date: 2024-05-23 DOI:10.1016/j.talanta.2024.126281

Zhuo Shi, Kaiwen Li, Yuwei Wang, Yuhan Hu, Zhanhong Li, Zhigang Zhu

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Abstract

In the medical field, changes in interleukin-6 (IL-6) concentration serve as essential biomarkers for monitoring and diagnosing various conditions, including acute inflammatory responses such as those seen in trauma and burns, and chronic illnesses like cancer. This paper detailed a label-free electrochemical aptamer sensor designed for IL-6 quantification. A composite material consisting of Ti₃C₂T_x and MoS₂ was successfully synthesized to fabricate this sensor. The synergistic effect of MoS₂'s catalytic action on hydrogen peroxide (H₂O₂), used as a signalling marker, when combined with the exceptional conductivity and large specific surface area of Ti₃C₂T_x, not only enables an increased loading of MoS₂ but also significantly boosts the electrochemical response. The in situ-reduced Au NPs provided stable immobilization sites for DNA aptamers (DNA_apt) and facilitated electron transfer, ensuring accurate IL-6 recognition. Under optimal conditions, the aptamer sensor exhibited a wide linear range (5 pg/mL to 100 ng/mL) and a low limit of detection (LOD) of 2.9 pg/mL. Its sensing performance in human serum samples highlights its potential as a promising clinical analysis tool.

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创新的无标记电化学适配体传感器：利用 Ti3C2Tx/MoS2/Au NPs 精确检测白细胞介素-6。

在医学领域，白细胞介素-6（IL-6）浓度的变化是监测和诊断各种疾病的重要生物标志物，包括创伤和烧伤等急性炎症反应以及癌症等慢性疾病。本文详细介绍了一种用于定量检测 IL-6 的无标记电化学适配体传感器。该传感器成功合成了一种由 Ti3C2Tx 和 MoS2 组成的复合材料。MoS2 对作为信号标记的过氧化氢（H2O2）的催化作用与 Ti3C2Tx 的优异导电性和大比表面积相结合，不仅能增加 MoS2 的负载量，还能显著提高电化学响应。原位还原的金纳米粒子为 DNA 合体（DNAapt）提供了稳定的固定位点，促进了电子传递，确保了对 IL-6 的准确识别。在最佳条件下，适配体传感器的线性范围很宽（5 pg/mL 至 100 ng/mL），检测限（LOD）低至 2.9 pg/mL。该传感器在人体血清样本中的传感性能突出表明了它作为一种有前途的临床分析工具的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.