Some Amino Acids Affect the Response of Grape (Vitis vinifera L.) Single Nodules In Vitro Multiplication

Q4 Agricultural and Biological Sciences Basrah Journal of Agricultural Sciences Pub Date : 2024-05-18 DOI:10.37077/25200860.2024.37.1.12
Waad S. Faizy, A. Z. K. Bashi, R. Toma
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Abstract

Organic nitrogen, which comes from amino acids, is absorbed and transported more rapidly than inorganic nitrogen by plant cells and tissues. Therefore, this study was conducted to evaluate the effect of some amino acids on the response of single nodes of grape Vitis vinifera L. Superior and Red globe cultivars were classified for in vitro multiplication to develop an efficient protocol for propagation of these two valuable species. The study included two experiments: first, the effect of adding three concentrations (0, 0.5, and 1) mg L-1 of Benzyl Adenine (BA) to the MS medium in the initiation stage. The second investigation utilized three amino acid types (glutamine, asparagine, and methionine) at five concentrations (0, 10, 20, 40, and 80) mg L-1 added to MS medium containing 2 mg L-1 of BA. Results indicated that the most significant newly initiated shoots (2.40 and 2.88) and the largest leaves number (7.90 and 5.88) for both cultivars, Superior and Red globe, respectively were obtained in a medium with 1 mg L-1 of BA. The multiplication results in eight weeks after cultivation showed that adding amino acid glutamine at 10 mg L-1 significantly increased the number of shoots/explant (6.60 and 6.00) and (29.50 and 38.30) leaves/explant for Red globe and Superior, respectively. The high amino acid concentrations (40 and 80) mg L-1 significantly reduced the studied growth parameters, especially the number of newly formed shoots. The results obtained from the present study exhibited the possibility of applying this modified protocol to the propagation of selected grape cultivars to encourage the expansion of the grape-growing industry in Iraq.
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某些氨基酸会影响葡萄(Vitis vinifera L.)单瘤体外繁殖的反应
来自氨基酸的有机氮比无机氮更快地被植物细胞和组织吸收和运输。因此,本研究评估了一些氨基酸对葡萄单节反应的影响,并对葡萄品种 Superior 和 Red globe 进行了体外繁殖分类,以制定这两个珍贵品种的高效繁殖方案。研究包括两项实验:第一项是在起始阶段向 MS 培养基中添加三种浓度(0、0.5 和 1)mg L-1 的苄基腺嘌呤(BA)的影响。第二项调查是在含有 2 毫克/升 BA 的 MS 培养基中添加五种浓度(0、10、20、40 和 80)毫克/升的三种氨基酸(谷氨酰胺、天冬酰胺和蛋氨酸)。结果表明,在含有 1 mg L-1 BA 的培养基中,Superior 和 Red globe 两个品种分别获得了最显著的新芽数(2.40 和 2.88)和最多的叶片数(7.90 和 5.88)。培养八周后的繁殖结果表明,添加 10 mg L-1 的氨基酸谷氨酰胺可显著增加红球和优等的芽/植株数(分别为 6.60 和 6.00)和叶/植株数(分别为 29.50 和 38.30)。高浓度氨基酸(40 和 80)毫克/升明显降低了所研究的生长参数,尤其是新芽数量。本研究的结果表明,有可能将这一改良方案用于繁殖选定的葡萄栽培品种,以促进伊拉克葡萄种植业的发展。
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来源期刊
Basrah Journal of Agricultural Sciences
Basrah Journal of Agricultural Sciences Environmental Science-Pollution
CiteScore
1.20
自引率
0.00%
发文量
35
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