Species authentication of Panax ginseng C.A. Mey. and ginseng extracts using mitochondrial nad2 intron 4 region

IF 3.8 2区 农林科学 Q1 PLANT SCIENCES Journal of Applied Research on Medicinal and Aromatic Plants Pub Date : 2024-05-01 DOI:10.1016/j.jarmap.2024.100554
Yuxue Pang, Xiaoran Tian, Dandan Wang, Hongtao Wang
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Abstract

Species authentication of Panax ginseng C.A. Mey. and its commercial products is vital for ensuring its therapeutic efficacy and food safety. To address the limitations of previous DNA methods in identifying ginseng products with severe DNA degradation, a multiplex PCR assay was established based on the single nucleotide polymorphism (SNP) markers exploited from the mitochondrial nad2 intron 4 region. This assay, which requires neither expensive equipment nor sequence analysis of PCR products, can detect 0.1% adulteration of P. quinquefolius or P. notoginseng down to a 0.001 ng level of template DNA. The developed assay proved effective in authenticating the botanical origin of commercial ginseng extracts without generating false-negative results under traditional PCR conditions. Therefore, the present study provides a valuable technical reference for regulating adulteration in commercial ginseng products, and the methodology can be applied in species authentication of other herbal products.

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利用线粒体 nad2 内含子 4 区鉴定人参 C.A. Mey.和人参提取物的种类
人参及其商业产品的物种鉴定对于确保其疗效和食品安全至关重要。为了解决以往 DNA 方法在鉴定 DNA 降解严重的人参产品方面的局限性,我们建立了一种基于线粒体 nad2 内含子 4 区单核苷酸多态性(SNP)标记的多重 PCR 检测方法。这种检测方法既不需要昂贵的设备,也不需要对 PCR 产物进行序列分析,就能检测出 0.1% 的五加皮或五加参掺假,其模板 DNA 含量可低至 0.001 ng。在传统的 PCR 条件下,所开发的检测方法可有效鉴定商品人参提取物的植物来源,而不会产生假阴性结果。因此,本研究为规范商品人参产品的掺假行为提供了有价值的技术参考,该方法也可应用于其他草药产品的物种鉴定。
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来源期刊
Journal of Applied Research on Medicinal and Aromatic Plants
Journal of Applied Research on Medicinal and Aromatic Plants Pharmacology, Toxicology and Pharmaceutics-Drug Discovery
CiteScore
6.40
自引率
7.70%
发文量
80
审稿时长
41 days
期刊介绍: JARMAP is a peer reviewed and multidisciplinary communication platform, covering all aspects of the raw material supply chain of medicinal and aromatic plants. JARMAP aims to improve production of tailor made commodities by addressing the various requirements of manufacturers of herbal medicines, herbal teas, seasoning herbs, food and feed supplements and cosmetics. JARMAP covers research on genetic resources, breeding, wild-collection, domestication, propagation, cultivation, phytopathology and plant protection, mechanization, conservation, processing, quality assurance, analytics and economics. JARMAP publishes reviews, original research articles and short communications related to research.
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