Yuxue Pang, Xiaoran Tian, Dandan Wang, Hongtao Wang
{"title":"Species authentication of Panax ginseng C.A. Mey. and ginseng extracts using mitochondrial nad2 intron 4 region","authors":"Yuxue Pang, Xiaoran Tian, Dandan Wang, Hongtao Wang","doi":"10.1016/j.jarmap.2024.100554","DOIUrl":null,"url":null,"abstract":"<div><p>Species authentication of <em>Panax ginseng</em> C.A. Mey. and its commercial products is vital for ensuring its therapeutic efficacy and food safety. To address the limitations of previous DNA methods in identifying ginseng products with severe DNA degradation, a multiplex PCR assay was established based on the single nucleotide polymorphism (SNP) markers exploited from the mitochondrial <em>nad</em>2 intron 4 region. This assay, which requires neither expensive equipment nor sequence analysis of PCR products, can detect 0.1% adulteration of <em>P. quinquefolius</em> or <em>P. notoginseng</em> down to a 0.001 ng level of template DNA. The developed assay proved effective in authenticating the botanical origin of commercial ginseng extracts without generating false-negative results under traditional PCR conditions. Therefore, the present study provides a valuable technical reference for regulating adulteration in commercial ginseng products, and the methodology can be applied in species authentication of other herbal products.</p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":null,"pages":null},"PeriodicalIF":3.8000,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Research on Medicinal and Aromatic Plants","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214786124000275","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Species authentication of Panax ginseng C.A. Mey. and its commercial products is vital for ensuring its therapeutic efficacy and food safety. To address the limitations of previous DNA methods in identifying ginseng products with severe DNA degradation, a multiplex PCR assay was established based on the single nucleotide polymorphism (SNP) markers exploited from the mitochondrial nad2 intron 4 region. This assay, which requires neither expensive equipment nor sequence analysis of PCR products, can detect 0.1% adulteration of P. quinquefolius or P. notoginseng down to a 0.001 ng level of template DNA. The developed assay proved effective in authenticating the botanical origin of commercial ginseng extracts without generating false-negative results under traditional PCR conditions. Therefore, the present study provides a valuable technical reference for regulating adulteration in commercial ginseng products, and the methodology can be applied in species authentication of other herbal products.
期刊介绍:
JARMAP is a peer reviewed and multidisciplinary communication platform, covering all aspects of the raw material supply chain of medicinal and aromatic plants. JARMAP aims to improve production of tailor made commodities by addressing the various requirements of manufacturers of herbal medicines, herbal teas, seasoning herbs, food and feed supplements and cosmetics. JARMAP covers research on genetic resources, breeding, wild-collection, domestication, propagation, cultivation, phytopathology and plant protection, mechanization, conservation, processing, quality assurance, analytics and economics. JARMAP publishes reviews, original research articles and short communications related to research.