Size Matters: Influence of Particle Size on Antioxidant, β-Glucan and Anti-inflammatory Potential in Pleurotus floridanus

IF 1.4 4区 生物学 Q4 MYCOLOGY International journal of medicinal mushrooms Pub Date : 2024-05-01 DOI:10.1615/intjmedmushrooms.2024054164
Wei Yan Wong, Siti Marjiana Ismail, Chia Wei Phan, Yee Shin Tan
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Abstract

Cellular damage resulting from elevated levels of free radicals can lead to persistent health issues. Pleurotus floridanus, an edible white oyster mushroom, is rich in β-glucans with potent antioxidant and anti-inflammatory properties. In this research, we examined the β-glucan content, total phenolic content, as well as antioxidant and anti-inflammatory potential of hot water extracts with varying particle sizes (<75 µm, 75-154 µm, 154-300 µm, and 300-600 µm) of both whole (WFB) and sliced (SFB) fruiting bodies of P. floridanus. The findings revealed that the β-glucan content increased as the particle size increased, though no significant differences were observed. Conversely, smaller particle sizes (<75 µm) of whole and sliced fruiting bodies of P. floridanus exhibited higher phenolic content, DPPH and ABTS scavenging activity and reducing ability compared to larger particle size (>75 µm). Out of the four samples (AW2, AW3, AS1, and AS2) with the highest antioxidant activity, selected for anti-inflammatory assays, all demonstrated the ability to reduce NO and TNF-α levels but did not enhance IL-10 expression in LPS-stimulated RAW264.7 cells. Interestingly, particle size < 75 to 300 µm did not appear to influence the anti-inflammatory activity, as no significant differences were observed among the particle sizes. Therefore, it is suggested that the particle size <300 µm of P. floridanus hot water extract could serve as a valuable source of antioxidant and anti-inflammatory compounds to counteract the harmful effects of free radicals.
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大小很重要:颗粒大小对花叶茄抗氧化、β-葡聚糖和抗炎潜力的影响
自由基水平升高造成的细胞损伤会导致持续的健康问题。杏鲍菇是一种可食用的白色杏鲍菇,富含β-葡聚糖,具有强大的抗氧化和抗炎特性。在这项研究中,我们检测了不同粒径(75 微米、75-154 微米、154-300 微米和 300-600 微米)的热水提取物的β-葡聚糖含量、总酚含量以及抗氧化和抗炎潜力。研究结果表明,β-葡聚糖含量随着粒径的增加而增加,但没有观察到显著差异。相反,与较大粒径(75 微米)的果实相比,较小粒径(75 微米)的花叶豚草完整子实体和切片子实体表现出更高的酚含量、DPPH 和 ABTS 清除活性和还原能力。在抗氧化活性最高的四个样品(AW2、AW3、AS1 和 AS2)中,被选作抗炎试验的所有样品都显示出降低 NO 和 TNF-α 水平的能力,但并不增强 LPS 刺激的 RAW264.7 细胞中 IL-10 的表达。有趣的是,粒径为 75 至 300 µm 的颗粒似乎并不影响抗炎活性,因为不同粒径的颗粒之间没有明显差异。因此,人们认为粒径为 300 微米的花叶蕨类植物热水提取物可作为抗氧化和抗炎化合物的重要来源,以抵消自由基的有害影响。
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来源期刊
CiteScore
2.60
自引率
16.70%
发文量
91
审稿时长
6-12 weeks
期刊介绍: The rapid growth of interest in medicinal mushrooms research is matched by the large number of disparate groups that currently publish in a wide range of publications. The International Journal of Medicinal Mushrooms is the one source of information that will draw together all aspects of this exciting and expanding field - a source that will keep you up to date with the latest issues and practice. The International Journal of Medicinal Mushrooms published original research articles and critical reviews on a broad range of subjects pertaining to medicinal mushrooms, including systematics, nomenclature, taxonomy, morphology, medicinal value, biotechnology, and much more.
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