Purification and Characterization of α-Mannosidase from Onion, Allium cepa.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-04-20 eCollection Date: 2024-01-01 DOI:10.5458/jag.jag.JAG-2023_0010

Yui Narita, Yota Tatara, Shigeki Hamada, Kaoru Kojima, Shuai Li, Takashi Yoshida

引用次数: 0

Abstract

α-Mannosidase (ALMAN) extracted from onion (Allium cepa) was purified by column chromatography such as hydrophobic and gel filtration. ALMAN is an acidic α-mannosidase that exhibits maximum activity against pNP-α-Man at pH 4.0-5.0 at 50°C. Amino acid sequence analysis of ALMAN was consistent with α-mannosidase deduced from Allium cepa transcriptome analysis. The gene alman was amplified by PCR using mRNA extracted from onions, and a full-length gene of 3,054 bp encoding a protein of 1,018 amino acid residues was revealed. ALMAN is classified as Glycoside Hydrolase Family (GH) 38 and showed homology with other plant-derived α-mannosidases such as tomato and hot pepper.

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洋葱（Allium cepa）中 α-甘露糖苷酶的纯化和特性。

从洋葱（Allium cepa）中提取的α-甘露糖苷酶（ALMAN）是通过疏水和凝胶过滤等柱层析方法纯化的。ALMAN 是一种酸性 α-甘露糖苷酶，在 pH 值为 4.0-5.0 和 50°C 时对 pNP-α-Man 的活性最高。ALMAN 的氨基酸序列分析与薤白转录组分析推导出的α-甘露糖苷酶一致。利用从洋葱中提取的 mRNA，通过 PCR 扩增了 ALMAN 基因，并发现了一个长达 3,054 bp 的全长基因，编码 1,018 个氨基酸残基的蛋白质。ALMAN 被归类为糖苷水解酶家族（GH）38，与番茄和辣椒等其他植物来源的 α-甘露糖苷酶存在同源性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.