The Better Visualization of the Highest Resistant Induction to Myzus persicae in Wild crucifer, Rorippa indica

Abstract

Rorippa indica, a wild Crucifer is already reported as resistant host to aphid. This wild crucifer draws an attention for molecular plant-aphid interaction biology to address the resistance mechanism as there is no resistant source in the Brassicaceae family to aphid proliferation. Before addressing the resistance, identification of the highest level of resistance is required. In the present study, identification of the highest resistant reaction to the aphid proliferation is focused from Rorippa indica -Myzus persicae interaction. Moreover, another objective is to identify the parameter for better visualization of the highest resistant reaction to Myzus persicae. Thus, three different aphids’ release like 5, 10 and 20 Myzus persicae were released in each foliage and monitoring the total aphid proliferation, total progeny proliferation, marginal progeny proliferation in 24, 48 and 72 h time point. Three elevated doses of aphid inoculums were explored to test the first hypothesis on whether the progeny production was dependent on an initial aphid density or not. The second hypothesis was on whether the host resistance induction was dependent on an initial aphid density or not. The third hypothesis was formulated on whether there was any differential ‘footprint’ developed in aphid herbivore vascular sap from the highest level of host resistance or not.