Enhancing starch hydrolysis in sweet potato (Ipomoea batatas (L.) Lam.) through CaCl2 solution mashing: insights into endogenous amylase activity

Marina Fernanda da Silva Junges, Daniele Bach, Luiz Gustavo Lacerda, Aline Alberti, Alessandro Nogueira, Ivo Mottin Demiate
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Abstract

Sweet potato (Ipomoea batatas (L.) Lam.) is a tuber root crop with high economical potential and China is responsible for harvesting roughly 70% of the world production. It is relatively easy to cultivate and has high nutritional value, including fiber, vitamins, starch and sugars. Cooking the roots results in deep changes of the carbohydrate profile due to the presence of active endogenous amylases. The objective of the present study was to evaluate the influence of CaCl2 mashing at different temperature-time conditions on the starch hydrolysis by endogenous amylases of sweet potato in order to suggest potential industrial applications. Three thermal treatments were performed with the aid of a Rapid Visco Analyzer (RVA), which allowed programming temperature-time profiles. After the treatments, the sugar analysis was carried out by high performance liquid chromatography (HPLC). Total starch content was measured in the insoluble residue from the hydrolysis and the hydrolysis efficiency was calculated. The results have demonstrated that the presence of CaCl2 enhanced enzyme activity, resulting in higher sugar yields when compared to hydrolysis conducted without CaCl2. The results from the present study are promising to the use of sweet potato to produce bioethanol, maltose syrup and vinegar.

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通过 CaCl2 溶液捣碎提高甘薯(Ipomoea batatas (L.) Lam.)的淀粉水解能力:对内源性淀粉酶活性的认识
甘薯(Ipomoea batatas (L.) Lam.)是一种经济潜力巨大的块根作物,中国的产量约占世界总产量的 70%。它比较容易种植,营养价值高,包括纤维、维生素、淀粉和糖。由于存在活性内源淀粉酶,烹饪根茎会导致碳水化合物谱发生深刻变化。本研究的目的是评估在不同温度-时间条件下 CaCl2 糖化对甘薯内源淀粉酶水解淀粉的影响,从而提出潜在的工业应用建议。借助快速粘度分析仪(RVA)进行了三次热处理,该分析仪可对温度-时间曲线进行编程。处理后,采用高效液相色谱法(HPLC)进行糖分分析。在水解后的不溶残留物中测量了总淀粉含量,并计算了水解效率。结果表明,与不加 CaCl2 的水解相比,CaCl2 的存在增强了酶的活性,从而提高了糖的产量。本研究的结果有望用于利用甘薯生产生物乙醇、麦芽糖浆和醋。
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