Antioxidant content following fermentation of lemongrass for herbal beverage development

Siti Madihah Don, Masmunira Rambli, Beston Faiek Nore
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Abstract

Consumers have increasingly favoured fermented drinks due to their high content of probiotic secondary metabolites. These beverages are believed to possess the capacity to safeguard against non-communicable ailments such as coronary heart disease, cancer, diabetes, antimicrobial infections, and other dietary-related disorders. Lemongrass (Cymbopogon citratus) is a commonly used botanical ingredient in therapeutic tea production. It is renowned for its highly valuable essential oil, which has significant commercial demand. This study examines the functional content and antioxidant effects of fermented beverages derived from lemongrass. We employed the yeast Saccharomyces cerevisiae to carry out the fermentation process on the lemongrass compositions, extending the duration from t = 24 to t = 96 h. We used non-fermented samples as control. This investigation identified numerous active biomolecules and polyphenols in the fermented samples, including flavonoids, tannins, cardiac glycosides, coumarins, terpenoids, steroids, saponins, and reducing sugars. After t = 24 h fermentation, the radical-scavenging activity reached its maximum level of 89.1%, and the antioxidant content reached 13.06 µg/ml, which is equivalent to the amount of ascorbic acid. After t = 36 h fermentation, the total phenolic content reached a concentration of 237.19 µg/ml, while the flavonoid content reached its peak at 55.21 µg/ml after t = 72 h fermentation. Lemongrass fermentation exhibits a wide range of phytochemicals and bioactive components that effectively eliminate free radicals, despite the antioxidant content fluctuation throughout the fermentation period of t = 24 to t = 96 h.

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柠檬草发酵后的抗氧化剂含量用于草药饮料开发
由于发酵饮料含有大量益生菌次级代谢物,消费者越来越青睐它们。这些饮料被认为具有预防非传染性疾病的能力,如冠心病、癌症、糖尿病、抗菌感染和其他与饮食有关的疾病。柠檬草(Cymbopogon citratus)是治疗茶生产中常用的植物成分。它以价值极高的精油而闻名,商业需求量很大。本研究探讨了柠檬草发酵饮料的功能含量和抗氧化效果。我们使用酵母菌对柠檬草成分进行发酵,发酵时间从 t = 24 小时延长到 t = 96 小时。这项调查在发酵样品中发现了许多活性生物大分子和多酚,包括类黄酮、单宁、强心甙、香豆素、萜类、甾体、皂苷和还原糖。发酵 t = 24 小时后,自由基清除活性达到最高水平,为 89.1%,抗氧化剂含量达到 13.06 µg/ml,相当于抗坏血酸的含量。发酵 t = 36 小时后,总酚含量达到 237.19 微克/毫升,黄酮含量在发酵 t = 72 小时后达到峰值,为 55.21 微克/毫升。在 t = 24 到 t = 96 小时的整个发酵过程中,尽管抗氧化剂含量有波动,但柠檬草发酵过程中表现出的多种植物化学物质和生物活性成分能有效消除自由基。
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