Optimization of ultrasonic extraction of bioactive components from Alpiniae oxyphyllae Fructus using response surface methodology

IF 3.8 2区农林科学 Q1 PLANT SCIENCES Journal of Applied Research on Medicinal and Aromatic Plants Pub Date : 2024-05-01 DOI:10.1016/j.jarmap.2024.100557

Yuyi Liu , Ruimin Wang , Xuan Hu , Chengfeng Yu , Zexin Wang , Lin Zhang , Sixin Liu , Congfa Li

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Abstract

Alpinia oxyphylla Miq. (A. oxyphylla) is a favorable medicinal and edible plant, its fructus has various biological activities. In the present study, ultrasonic extraction (UE) of the active constituents from Alpiniae oxyphyllae Fructus (A. oxyphyllae Fructus) was optimized using central composite design and the extract was characterized for its activities. The results suggested that under optimum UE conditions (ethanol concentration (60%, v/v), solid-liquid ratio (1:40), and number of extractions (4)), the total phenolic content (TPC), total flavonoid content (TFC), and total triterpene content (TTC) were 11.92 mg GAE/g DW, 15.37 mg RE/g DW, and 58.04 mg UAE/g DW, respectively. The 8 major compounds in A. oxyphyllae Fructus extract were identified and quantified by ultra-performance liquid chromatography tandem electrospray ionization time-of-flight mass spectrometry (UPLC-ESI-QTrap-MS) and high performance liquid chromatography (HPLC). The extract of A. oxyphyllae Fructus obtained by UE based on optimal parameters had higher TPC, TFC and TTC, better antioxidant activities, antibacterial activities and enzyme inhibitory activities as compared to boiling water extraction (BWE) and maceration extraction (ME).

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利用响应面方法优化超声波提取山银花中的生物活性成分

米克(）是一种药用和食用植物，其果实具有多种生物活性。在本研究中，采用中心复合设计对超声波萃取（UE）果实（Fructus）中的活性成分进行了优化，并对萃取物的活性进行了表征。结果表明，在最佳超声萃取条件下（乙醇浓度（60%，v/v）、固液比（1:40）和萃取次数（4）），总酚含量（TPC）、总黄酮含量（TFC）和总三萜含量（TTC）分别为 11.92 mg GAE/g DW、15.37 mg RE/g DW 和 58.04 mg UAE/g DW。采用超高效液相色谱-串联电喷雾飞行时间质谱法（UPLC-ESI-QTrap-MS）和高效液相色谱法（HPLC）对果实提取物中的 8 种主要化合物进行了鉴定和定量。与沸水提取（BWE）和浸渍提取（ME）相比，基于最佳参数的 UE 提取物具有更高的 TPC、TFC 和 TTC，以及更好的抗氧化活性、抗菌活性和酶抑制活性。

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来源期刊

Journal of Applied Research on Medicinal and Aromatic Plants Pharmacology, Toxicology and Pharmaceutics-Drug Discovery

CiteScore

6.40

自引率

7.70%

发文量

审稿时长

41 days

期刊介绍： JARMAP is a peer reviewed and multidisciplinary communication platform, covering all aspects of the raw material supply chain of medicinal and aromatic plants. JARMAP aims to improve production of tailor made commodities by addressing the various requirements of manufacturers of herbal medicines, herbal teas, seasoning herbs, food and feed supplements and cosmetics. JARMAP covers research on genetic resources, breeding, wild-collection, domestication, propagation, cultivation, phytopathology and plant protection, mechanization, conservation, processing, quality assurance, analytics and economics. JARMAP publishes reviews, original research articles and short communications related to research.