Molecular Characterization and Haplotype Diversity of Aporcelaimellus obtusicaudatus (Bastian, 1865) Altherr, 1968 (Dorylaimida, Aporcelaimidae) from India

IF 0.5 4区生物学 Q4 BIOLOGY Biology Bulletin Pub Date : 2024-05-31 DOI:10.1134/s1062359023604378

Himani Sharma, Ashok Kumar Chaubey, Ebrahim Shokoohi

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Abstract

Aporcelaimellus obtusicaudatus was recovered from mustard and sugarcane fields of Uttar Pradesh and Uttarakhand, India. The present species was identified through morphology, molecular and phylogenetic analysis. The Indian populations are characterized by having females with a body length (1.7–3.4 mm), lip region (4.9–8.8 µm), Amphids 6–9 µm, odontostyle length (15.7–26.4 µm), neck length (374.6–745.1 µm), pharyngeal expansion occupying 45–60% of neck length, simple uterus (67.4–124.3 µm), V = 49.1–58.7, female tail (32–44.9 µm, c = 41.1–105, c' = 0.5–1.3) with short, conoid, rounded terminus to convex conical. The molecular phylogenetic analysis based on 18S and 28S rDNA revealed A. obtusicaudatus as a paraphyly. The present populations of Indian A. obtusicaudatus (OM269524; OP020675) placed close to deposited Indian sequence (OP048816; OP048823) and Slovakian populations of A. obtusicaudatus (MH206119). The result of 28S rDNA phylogeny showed that the present population (OM258706) placed close to other populations of the same species from India (OM418794) with 0.55 posterior probability supports. The haplotype analysis based on 28S rDNA revealed 6 haplotypes, which displayed low nucleotide (π = 0.013) diversity. In contrast, haplotype diversity (Hd) was 1.00, and the number of segregating sites (S) was 14. The phylogenetic analysis grouped the various populations into two clades, and the result showed German populations placed into two clades, which showed high variability in haplotype supported by the haplotype network. Tajima (D) and Fu’s Fs were found to be negative (P > 0.05), whereas the overall FST value was 0.31040 (P = 0.118). In conclusion, genetic analysis revealed 31.04% variation among groups/populations and 68.95 within populations, indicating a low genetic variation between the same populations of A. obtusicaudatus belonging to the same country.

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印度 Aporcelaimellus obtusicaudatus (Bastian, 1865) Altherr, 1968 (Dorylaimida, Aporcelaimidae) 的分子特征和单倍型多样性

摘要在印度北方邦和北阿坎德邦的芥菜田和甘蔗田中发现了钝顶脐虫（Aporcelaimellus obtusicaudatus）。通过形态学、分子和系统发育分析确定了该物种。印度种群的特征是雌性体长（1.7-3.4 毫米）、唇区（4.9-8.8 微米）、虹膜 6-9 微米、尾柄长（15.7-26.4 微米）、颈长（374.6-745.1 µm），咽部膨大占颈长的 45-60%，单子宫（67.4-124.3 µm），V = 49.1-58.7，雌尾（32-44.9 µm，c = 41.1-105，c' = 0.5-1.3）短，圆锥形，末端圆至凸圆锥形。基于 18S 和 28S rDNA 的分子系统学分析表明，A. obtusicaudatus 属于旁系。目前的印度 A. obtusicaudatus 种群（OM269524；OP020675）与已保存的印度序列（OP048816；OP048823）和斯洛伐克 A. obtusicaudatus 种群（MH206119）相近。28S rDNA 系统进化结果表明，本种群（OM258706）与印度的其他同种种群（OM418794）相近，后验概率为 0.55。基于 28S rDNA 的单倍型分析发现了 6 个单倍型，其核苷酸（π = 0.013）多样性较低。相比之下，单倍型多样性（Hd）为 1.00，分离位点数（S）为 14。系统发生分析将不同种群分为两个支系，结果显示德国种群被分为两个支系，单倍型网络支持的单倍型变异性较高。Tajima（D）和 Fu's Fs 均为负值（P >；0.05），而总体 FST 值为 0.31040（P = 0.118）。总之，遗传分析表明，群体/种群间的变异率为 31.04%，种群内的变异率为 68.95%，这表明属于同一国家的 A. obtusicaudatus 同一种群之间的遗传变异率较低。

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来源期刊

Biology Bulletin 生物-生物学

CiteScore

0.70

自引率

20.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Biology Bulletin (Izvestiya Rossiiskoi Akademii Nauk – Seriya Biologicheskaya) is an interdisciplinary journal of general biology. It focuses on fundamental studies in the fields of cell biology, biochemistry, zoology, botany, physiology, and ecology. This journal publishes current materials of experimental studies and surveys on current problems in general biology. It also publishes information on scientific conferences and new books in the fields of general biology.