Traditional Chinese Medicine Clerodendrum japonicum (C. japonicum) Ameliorates the Pulmonary Fibrosis through Inhibiting the TGF-β/Smad3 Signaling Pathway

IF 0.5 4区生物学 Q4 BIOLOGY Biology Bulletin Pub Date : 2024-05-31 DOI:10.1134/s1062359024600028

Jiangcun Wei, Chenglong Wang, Jianlong Zhou, Yunli Tang, Qingmei Deng, Hong Lei, Liping Qin, Zujie Qin

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Abstract

Clerodendrum japonicum (C. japonicum) is a classical Traditional Chinese Medicine (TCM) and has been used to treat the dozens of diseases in China. However, scientific evidence for the pulmonary fibrosis (PF) treatment of C. japonicum used in traditional medicine is lacking. This study evaluated whether C. japonicum ameliorates the PF and the potential molecular mechanism of C. japonicum treatment in vivo and in vitro. We used the PF rat model in vivo. The lung coefficient, HE and Masson staining were used to evaluate the lung tissues injury. Transmission electron microscope (TEM) was used to evaluate the organelle structural changes. The inflammatory cytokines IL-1β, IL-6, MCP-1 and TNF-α were detected using ELISA assay. ROS, MDA and GSH were measured to explore the oxidative stress. Western blotting, immunofluorescence (IF) and RT-qPCR were performed to observe the expressions of PF and TGF-β/Smad3 signaling pathway related genes and proteins. Firstly, C. japonicum could effectively alleviate the PF injury in rat model. C. japonicum could effectively inhibit the levels of inflammatory factors in PF animal model, at the same time, C. japonicum could reasonably regulate oxidative stress factors in PF animal model. Furthermore, TGF-β/Smad3 signaling pathway was involved in the PF animal model and C. japonicum could inhibit the TGF-β/Smad3 signaling pathway in PF treatment effectively. C. japonicum might attenuate the PF in the PF rat model in vivo through inhibiting the TGF-β/Smad3 signaling pathway. These findings suggested that C. japonicum might act as a promising TCM to treat the PF.

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中药日本细辛通过抑制 TGF-β/Smad3 信号通路改善肺纤维化

摘要日本鸦胆子（Clerodendrum japonicum，简称C.japonicum）是一种经典中药，在中国被用于治疗数十种疾病。然而，传统医学中使用的日本白术治疗肺纤维化（PF）缺乏科学证据。本研究评估了日本忍冬是否能改善肺纤维化以及日本忍冬在体内和体外治疗肺纤维化的潜在分子机制。我们在体内使用了 PF 大鼠模型。采用肺系数、HE和Masson染色来评估肺组织损伤。透射电子显微镜（TEM）用于评估细胞器结构的变化。用 ELISA 法检测炎症细胞因子 IL-1β、IL-6、MCP-1 和 TNF-α。通过测量 ROS、MDA 和 GSH 来探究氧化应激。采用 Western 印迹、免疫荧光（IF）和 RT-qPCR 技术观察 PF 和 TGF-β/Smad3 信号通路相关基因和蛋白的表达。结果表明：首先，忍冬藤能有效缓解大鼠模型的PF损伤。日本忍冬酰胺能有效抑制 PF 动物模型中炎症因子的水平，同时日本忍冬酰胺还能合理调节 PF 动物模型中的氧化应激因子。此外，TGF-β/Smad3 信号通路也参与了 PF 动物模型的研究，而日本忍冬藤能在 PF 治疗中有效抑制 TGF-β/Smad3 信号通路。日本茨实菌可能通过抑制TGF-β/Smad3信号通路来减轻体内PF大鼠模型中的PF。这些研究结果表明，日本鸦胆子可能是一种很有前景的治疗PF的中药。

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来源期刊

Biology Bulletin 生物-生物学

CiteScore

0.70

自引率

20.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Biology Bulletin (Izvestiya Rossiiskoi Akademii Nauk – Seriya Biologicheskaya) is an interdisciplinary journal of general biology. It focuses on fundamental studies in the fields of cell biology, biochemistry, zoology, botany, physiology, and ecology. This journal publishes current materials of experimental studies and surveys on current problems in general biology. It also publishes information on scientific conferences and new books in the fields of general biology.