Comparison of stromal vascular fraction cell composition between Coleman fat and extracellular matrix/stromal vascular fraction gel.

IF 3.5 4区 生物学 Q2 ENDOCRINOLOGY & METABOLISM Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-06-03 DOI:10.1080/21623945.2024.2360037
Xiaoyun Li, Guohong Zhang, Mengmeng Wang, Changhao Lu, Guangping Zhang, Zhehui Chen, Yingchang Ji
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Abstract

As a mechanically condensed product of Coleman fat, extracellular matrix/stromal vascular fraction gel (ECM/SVF-gel) eliminates adipocytes, concentrates SVF cells, and improves fat graft retention. This study aims to compare SVF cell composition between Coleman fat and ECM/SVF-gel. Matched Coleman fat and ECM/SVF-gel of 28 healthy women were subjected to RNA-seq, followed by functional enrichment and cell-type-specific enrichment analyses, and deconvolution of SVF cell subsets, reconstructing SVF cell composition in the transcriptome level. ECM/SVF-gels had 9 upregulated and 73 downregulated differentially expressed genes (DEGs). Downregulated DEGs were mainly associated with inflammatory and immune responses, and enriched in fat macrophages. M2 macrophages, resting CD4+ memory T cells, M1 macrophages, resting mast cells, and M0 macrophages ranked in the top five most prevalent immune cells in the two groups. The proportions of the principal non-immune cells (e.g., adipose-derived stem cells, pericytes, preadipocytes, microvascular endothelial cells) had no statistical differences between the two groups. Our findings reveal ECM/SVF-gels share the same dominant immune cells beneficial to fat graft survival with Coleman fat, but exhibiting obvious losses of immune cells (especially macrophages), while non-immune cells necessary for adipose regeneration might have no significant loss in ECM/SVF-gels and their biological effects could be markedly enhanced by the ECM/SVF-gel's condensed nature.

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比较科尔曼脂肪和细胞外基质/基质血管部分凝胶的基质血管部分细胞组成。
细胞外基质/基质血管组分凝胶(ECM/SVF-gel)是 Coleman 脂肪的机械凝结产物,它能消除脂肪细胞,浓缩 SVF 细胞,提高脂肪移植的保留率。本研究旨在比较科尔曼脂肪和 ECM/SVF 凝胶的 SVF 细胞组成。对 28 名健康女性的匹配 Coleman 脂肪和 ECM/SVF 凝胶进行 RNA-seq,然后进行功能富集和细胞类型特异性富集分析,并对 SVF 细胞亚群进行解卷积,从而在转录组水平重建 SVF 细胞组成。ECM/SVF-凝胶有9个上调和73个下调的差异表达基因(DEGs)。下调的 DEGs 主要与炎症和免疫反应有关,并富集在脂肪巨噬细胞中。M2 巨噬细胞、静息 CD4+ 记忆 T 细胞、M1 巨噬细胞、静息肥大细胞和 M0 巨噬细胞位列两组中最常见免疫细胞的前五位。主要非免疫细胞(如脂肪源性干细胞、周细胞、前脂肪细胞、微血管内皮细胞)的比例在两组之间没有统计学差异。我们的研究结果表明,ECM/SVF-凝胶与 Coleman 脂肪具有相同的有利于脂肪移植物存活的优势免疫细胞,但免疫细胞(尤其是巨噬细胞)明显减少,而脂肪再生所需的非免疫细胞在 ECM/SVF 凝胶中可能没有明显减少,其生物效应可能因 ECM/SVF 凝胶的凝结特性而明显增强。
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来源期刊
Adipocyte
Adipocyte Medicine-Histology
CiteScore
6.50
自引率
3.00%
发文量
46
审稿时长
32 weeks
期刊介绍: Adipocyte recognizes that the adipose tissue is the largest endocrine organ in the body, and explores the link between dysfunctional adipose tissue and the growing number of chronic diseases including diabetes, hypertension, cardiovascular disease and cancer. Historically, the primary function of the adipose tissue was limited to energy storage and thermoregulation. However, a plethora of research over the past 3 decades has recognized the dynamic role of the adipose tissue and its contribution to a variety of physiological processes including reproduction, angiogenesis, apoptosis, inflammation, blood pressure, coagulation, fibrinolysis, immunity and general metabolic homeostasis. The field of Adipose Tissue research has grown tremendously, and Adipocyte is the first international peer-reviewed journal of its kind providing a multi-disciplinary forum for research focusing exclusively on all aspects of adipose tissue physiology and pathophysiology. Adipocyte accepts high-profile submissions in basic, translational and clinical research.
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