Thymol increases primordial follicle activation, protects stromal cells, collagen fibers and down-regulates expression of mRNA for superoxide dismutase 1, catalase and periredoxin 6 in cultured bovine ovarian tissues

IF 2.2 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Animal Reproduction Science Pub Date : 2024-05-29 DOI:10.1016/j.anireprosci.2024.107514
Francisco F. Caetano Filho , Lais R.F. Paulino , Vitória S. Bezerra , Venância A.N. Azevedo , Pedro A.A. Barroso , Francisco C. Costa , Geovany G. Amorim , José R.V. Silva
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Abstract

This study aims to investigate the influence of thymol on primordial follicle growth and survival, as well as on collagen fibers and stromal cells density in bovine ovarian tissues cultured in vitro. The activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), the thiol levels and the expression of mRNAs for SOD1, CAT, periredoxin 6 (PRDX6) and GPX1 were also investigated. Ovarian cortical tissues were cultured in α-MEM+ alone or with thymol (400, 800, 1600 or 3200 μg/mL) for six days. Before and after culture, the tissues were processed for histological analysis to evaluate follicular activation, growth, morphology, ovarian stromal cell density and collagen fibers. The levels of mRNA for SOD1, CAT, GPX1 and PRDX6 were evaluated by real-time PCR. The results show that tissues cultured with thymol (400 and 800 µg/mL) had increased percentages of normal follicles, when compared to tissues cultured in other treatments. At concentrations of 400 and 800 µg/mL, thymol maintained the rate of normal follicles similar to the uncultured control. In addition, 400 µg/mL thymol increased follicle activation, collagen fibers and stromal cell density of when compared to tissues cultured in control medium. The presence of 800 µg/mL thymol in culture medium increased CAT activity, while 400 or 800 µg/mL thymol reduced mRNA levels for SOD1, CAT and PRDX6, but did not alter GPX1 expression. In conclusion, 400 µg/mL thymol increases primordial follicle activation, preserves stromal cells, collagen fibers, and down-regulates expression of mRNA for SOD1, CAT and PRDX6 in cultured bovine ovarian tissues.

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百里酚可提高原始卵泡的活化,保护基质细胞和胶原纤维,并下调培养牛卵巢组织中超氧化物歧化酶 1、过氧化氢酶和过氧化物酶 6 的 mRNA 表达。
本研究旨在探讨百里酚对体外培养的牛卵巢组织中原始卵泡生长和存活率以及胶原纤维和基质细胞密度的影响。此外,还研究了超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GPX)的活性、硫醇水平以及 SOD1、CAT、过氧化酶 6(PRDX6)和 GPX1 的 mRNA 表达。卵巢皮质组织在α-MEM+中单独或与百里酚(400、800、1600 或 3200 μg/mL)一起培养六天。培养前后,对组织进行组织学分析,以评估卵泡活化、生长、形态、卵巢基质细胞密度和胶原纤维。实时 PCR 评估了 SOD1、CAT、GPX1 和 PRDX6 的 mRNA 水平。结果显示,与其他处理的组织相比,使用百里酚(400 和 800 µg/mL)培养的组织中正常卵泡的百分比有所增加。在400和800微克/毫升的浓度下,胸腺酚保持的正常卵泡率与未培养的对照组相似。此外,与在对照培养基中培养的组织相比,400微克/毫升的胸腺酚增加了卵泡活化、胶原纤维和基质细胞密度。培养基中含有800微克/毫升百里酚会增加CAT活性,而400或800微克/毫升百里酚会降低SOD1、CAT和PRDX6的mRNA水平,但不会改变GPX1的表达。总之,在培养的牛卵巢组织中,400 µg/mL 百里酚可增加原始卵泡的活化,保护基质细胞和胶原纤维,并下调 SOD1、CAT 和 PRDX6 的 mRNA 表达。
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来源期刊
Animal Reproduction Science
Animal Reproduction Science 农林科学-奶制品与动物科学
CiteScore
4.50
自引率
9.10%
发文量
136
审稿时长
54 days
期刊介绍: Animal Reproduction Science publishes results from studies relating to reproduction and fertility in animals. This includes both fundamental research and applied studies, including management practices that increase our understanding of the biology and manipulation of reproduction. Manuscripts should go into depth in the mechanisms involved in the research reported, rather than a give a mere description of findings. The focus is on animals that are useful to humans including food- and fibre-producing; companion/recreational; captive; and endangered species including zoo animals, but excluding laboratory animals unless the results of the study provide new information that impacts the basic understanding of the biology or manipulation of reproduction. The journal''s scope includes the study of reproductive physiology and endocrinology, reproductive cycles, natural and artificial control of reproduction, preservation and use of gametes and embryos, pregnancy and parturition, infertility and sterility, diagnostic and therapeutic techniques. The Editorial Board of Animal Reproduction Science has decided not to publish papers in which there is an exclusive examination of the in vitro development of oocytes and embryos; however, there will be consideration of papers that include in vitro studies where the source of the oocytes and/or development of the embryos beyond the blastocyst stage is part of the experimental design.
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