Fecal Microbiota Transplantation in a Domestic Ferret Suffering from Chronic Diarrhea and Maldigestion-Fecal Microbiota and Clinical Outcome: A Case Report.

IF 1.7 Q2 VETERINARY SCIENCES Veterinary medicine (Auckland, N.Z.) Pub Date : 2024-05-28 eCollection Date: 2024-01-01 DOI:10.2147/VMRR.S449473
Sean J Ravel, Victoria M Hollifield
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Abstract

This case report describes the effects of fecal microbiota transplantation (FMT) administered via enema in a 4-year-old spayed, champagne Domestic Ferret (Mustela putorius furo) with chronic diarrhea, maldigestion and weight loss. We aimed to establish a protocol for FMT as a novel therapeutic treatment for chronic diarrhea in domestic ferrets. We mapped the fecal microbiome by 16S rRNA gene amplicon sequencing to track the patient's fecal microbiota throughout the treatment and observation period. Initial oral FMTs were associated with temporary weight improvement but subsequent treatments, via enema and oral delivery, showed varied outcomes. Molecular analysis highlighted distinct gut microbiota composition profiles between the healthy donor and the diseased ferret. The diseased ferret initially exhibited high abundance of Enterobacteriaceae, Escherichia, and Enterobacter, which ultimately normalized to level like those found in the donor ferret. Overall, the gut microbiota of the recipient became more similar to the donor microbiota using a Yue-Clayton theta coefficients analysis. After a restoration of the gut microbiota and clinical improvement, the recipient's symptoms returned indicating that repeated FMTs might be required for long-term resolution of symptoms and complete restructuring of the gut microbiota. Future studies are warranted to map the microbiome of a larger population of domestic ferrets to investigate a potential correlation between fecal microbiota profiles and chronic/acute gastrointestinal disorders.

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慢性腹泻和消化不良家养雪貂的粪便微生物群移植--粪便微生物群与临床结果:病例报告。
本病例报告描述了对一只患有慢性腹泻、消化不良和体重减轻的 4 岁绝育香槟色家养雪貂(Mustela putorius furo)通过灌肠进行粪便微生物群移植(FMT)的效果。我们的目的是制定一种 FMT 方案,作为治疗家貂慢性腹泻的新型疗法。我们通过 16S rRNA 基因扩增片测序绘制了粪便微生物组图,以便在整个治疗和观察期间跟踪患者的粪便微生物群。最初的口服 FMT 与体重的暂时改善有关,但随后通过灌肠和口服给药进行的治疗显示出不同的结果。分子分析突显了健康供体和患病雪貂之间不同的肠道微生物群组成特征。患病雪貂最初表现出较高的肠杆菌科、大肠杆菌和肠杆菌含量,但最终正常化到与供体雪貂相同的水平。总体而言,通过 Yue-Clayton theta 系数分析,受体的肠道微生物群与供体的微生物群更加相似。在恢复肠道微生物群并改善临床症状后,受体的症状又出现了,这表明要长期缓解症状并完全重组肠道微生物群,可能需要反复进行 FMT。未来的研究有必要绘制更多家养雪貂的微生物组图谱,以调查粪便微生物组图谱与慢性/急性胃肠道疾病之间的潜在相关性。
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